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首页> 外文期刊>Infection and immunity >Evidence for the non-protein nature of the receptor for the enterotoxin in Vibrio cholerae on murine lymphoid cells.
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Evidence for the non-protein nature of the receptor for the enterotoxin in Vibrio cholerae on murine lymphoid cells.

机译：鼠淋巴细胞霍乱弧菌肠毒素受体的非蛋白质性质的证据。

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Lymphoid cells from A/J and BALB/c strains of mice were iodinated with 125I by the lactoperoxidase method and the plasma membranes were disrupted by freezing and thawing or with 0.5 percent Nonidet P-40, a nonionic detergent. Attempts to find choleragen reactive iodinated material in 0.5 percent Nonidet P-40 lysates were unsuccessful even when the cells were incubated with choleragen before lysis. Freezing and thawing the cells resulted in the release of iodinated choleragen reactive material. The interaction of choleragen with the iodinated material could be inhibited (at low choleragen concentrations) or enhanced (at high choleragen concentrations) by the addition of the ganglioside G-M1 to the the immune precipitation system. The results are consistent with the hypothesis that the receptor for choleragen is a glycolipid and reduce, but do not totally eliminate, the likelihood that the receptor is glycoprotein in nature.
机译：通过乳过氧化物酶方法用125 I碘化A / J和BALB / c小鼠的淋巴样细胞，并通过冷冻和融化或用0.5％的Nonidet P-40（一种非离子型洗涤剂）破坏质膜。尝试在0.5％的Nonidet P-40裂解物中发现霍乱素反应性碘化物质是不成功的，即使在裂解前将细胞与霍乱素一起孵育。冷冻和融化细胞会导致碘化霍乱原反应性物质的释放。通过在免疫沉淀系统中添加神经节苷脂G-M1，可以抑制（在低浓度的胆源激素下）或增强（在高浓度的胆源激素下）霍乱素与碘化物的相互作用。该结果与以下假设相一致：霍乱原的受体是糖脂，并且减少了但没有完全消除该受体本质上是糖蛋白的可能性。

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