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首页> 外文期刊>Infection and immunity >Isolation of skin permeability factors from culture filtrates of Salmonella typhimurium.
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Isolation of skin permeability factors from culture filtrates of Salmonella typhimurium.

机译：从鼠伤寒沙门氏菌培养滤液中分离皮肤通透性因子。

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Engerotoxins isolated from Vibrio cholerae and toxigenic Escherichia coli cause permeability alterations in rabbit skin. Firm induration and erythema are observed within 18 to 24 h, and visualization of the reaction may be enhanced by intravenous injection of Pontamine sky blue dye. Two skin permeability factors (PF) have been found in culture filtrates of Salmonella typhimurium. A rapid acting factor, produced optimally in brain heart infusion broth at 37 degrees C by numerous Salmonella species, has a critical bluing time of 1 h after completion of skin testing. This rapid PF is heat stable at 100 degrees C for at least 4 h and has no associated induration. The delayed factor is heat labile, being completly destroyed within 30 min at 75 degrees C and causes marked duration of the rabbit skin within 18 h that is indistinguishable from the permeability reactions of V. cholerae and E. coli enterotoxins. Induration produced by the delayed PF is observed only after chromatography of the culture filtrate on a Sephadex G-100 column. Thus, the effects of the delayed PF appear to be masked or blocked by an inhibitor-like substance present in crude culture filtrates. Both early and delayed factors are estimated to have a molecular weight of at least 90,000. It is postulated that one or both of these factors may participate in the pathogenesis of Salmonella infections.
机译：从霍乱弧菌和产毒性大肠杆菌分离出的Engerotoxins会引起兔皮肤通透性改变。在18至24小时内观察到硬结和红斑，并且通过静脉注射Pontamine天蓝色染料可以增强反应的可视化。在鼠伤寒沙门氏菌的培养滤液中发现了两种皮肤渗透因子（PF）。快速反应因子是由众多沙门氏菌在37℃的脑心浸液中最佳产生的，在完成皮肤测试后的关键上蓝时间为1小时。这种快速PF在100摄氏度下热稳定至少4小时，并且没有硬结。延迟因子是热不稳定的，在75摄氏度下在30分钟内被完全破坏，并在18 h内引起明显的兔子皮肤持续时间，这与霍乱弧菌和大肠杆菌肠毒素的渗透性反应没有区别。仅在将培养滤液在Sephadex G-100色谱柱上层析后，才能观察到由延迟PF产生的硬结。因此，延迟PF的作用似乎被粗培养滤液中存在的抑制剂样物质掩盖或阻断。早期和延迟因子的分子量均估计至少为90,000。假定这些因素中的一个或两个都可能参与沙门氏菌感染的发病机理。

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