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首页> 外文期刊>Infection and immunity >Interaction of Nocardia asteroides with cultured rabbit alveolar macrophages.
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Interaction of Nocardia asteroides with cultured rabbit alveolar macrophages.

机译：诺卡氏小行星与培养的兔肺泡巨噬细胞的相互作用。

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The interaction between virulent and less virulent strains of Nocardia asteroides and cultured rabbit alveolar macrophages was studied. It was shown that cells of the less virulent strain (N. asteroides 10905) were rapidly phagocytized and destroyed. However, some cells were able to avoid being killed, and they persisted within the macrophage in an altered, gram-negative form. These variants apparently increased in numbers after several days within the macrophage population, so that at 9 days postinfection more colony-forming units per macrophage were recovered than at 3 h. Little or no extracellular growth was observed in the tissue culture medium. During the increase at 9 days, both transitional-phase variants and L-forms of N. asteroides were isolated from the macrophages but not from the medium. Gram-positive bacterial cells were never observed in 9-day infected macrophages. In contrast, cells of the more virulent strain (N. asteroides 14759) were not destroyed after being ingested. After 6 h postinfection, it was observed that the number of colony-forming units per macrophage had increased significantly. There was no corresponding increase in extracellular organisms observed in the culture medium. Therefore, cells of N. asteroides 14759 were able to grow rapidly within cultured rabbit alveolar macrophages. Upon continued incubation of the infected cells (24 h postinfection), it was shown that this strain of Nocardia grew out of the macrophages as acid-fast branching filaments. From these data, it is clear that the initial interaction between N. asteroides and unstimulated, nonimmune alveolar macrophages depends upon the relative virulence of the nocardial strain.
机译：研究了诺卡氏小球藻的强毒株和弱毒株与培养的兔肺泡巨噬细胞之间的相互作用。结果表明，毒性较低的菌株（N. asteroides 10905）的细胞被迅速吞噬并破坏。但是，一些细胞能够避免被杀死，它们以改变的革兰氏阴性形式持续存在于巨噬细胞中。这些变体在巨噬细胞群中几天后的数量明显增加，因此在感染后第9天，每个巨噬细胞回收的菌落形成单位多于3小时。在组织培养基中观察到很少或没有细胞外生长。在第9天的增加过程中，从巨噬细胞中而不是从培养基中分离出小叶猪笼草的过渡期变体和L型。在感染9天的巨噬细胞中从未观察到革兰氏阳性细菌细胞。相比之下，更具强毒力的细胞（小行星猪笼草14759）被摄入后并未被破坏。感染后6小时后，观察到每个巨噬细胞集落形成单位的数量显着增加。在培养基中没有观察到相应的细胞外生物增加。因此，N。asteroides 14759的细胞能够在培养的兔肺泡巨噬细胞中快速生长。在继续孵育感染的细胞（感染后24小时）后，发现诺卡氏菌菌株从巨噬细胞中生长出来，成为耐酸的分支丝。从这些数据可以清楚地看出，小行星猪笼草和未刺激的非免疫肺泡巨噬细胞之间的初始相互作用取决于诺卡氏菌菌株的相对毒性。

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