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首页> 外文期刊>Infection and immunity >Immunological and chemical characterization of the extracellular antigens from Corynebacterium vaginale.
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Immunological and chemical characterization of the extracellular antigens from Corynebacterium vaginale.

机译：阴道棒状杆菌细胞外抗原的免疫学和化学表征。

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Supernatants from 72-h peptone-starch-dextrose broth cultures of Corynebacterium vaginale contained significant quantities of three extracellular, soluble antigens (ESA). The ESA were concentrated and partially purified by dialysis followed by ammonium sulfate and ethanol precipitation. Diethylaminoethylcellulose columns were used to isolate two of the three ESA. The ESA were shown to be similar to antigens found on whole C. vaginale cells. Absorption studies indicated that the cell antigens are located at, or near, the surface. On the bases of heat stability, resistance to protease treatment, concanavalin A binding activity, and susceptibility to periodate, it appears that all three ESA are polysaccharide or glycoprotein in nature.
机译：来自阴道棒状杆菌72小时蛋白ept-淀粉-葡萄糖肉汤培养物中的上清液含有大量的三种细胞外可溶性抗原（ESA）。浓缩ESA，并通过透析，然后用硫酸铵和乙醇沉淀进行部分纯化。二乙氨基乙基纤维素柱用于分离三个ESA中的两个。 ESA被证明类似于在整个阴道梭状芽胞杆菌阴道细胞中发现的抗原。吸收研究表明，细胞抗原位于表面或附近。基于热稳定性，对蛋白酶处理的抗性，伴刀豆球蛋白A的结合活性和对高碘酸盐的敏感性，似乎所有三种ESA本质上都是多糖或糖蛋白。

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