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首页> 外文期刊>Infection and immunity >In vitro colonization of Streptococcus mutans on enamel.
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In vitro colonization of Streptococcus mutans on enamel.

机译：变形链球菌在牙釉质上的体外定植。

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An in vitro model consisting of enamel from extracted human molars, suspended from wires in inoculated culture tubes, was used to study the adhesion of bacteria to enamel. Under conditions in which there was no macroscopically visible plaque formation, electron micrographs showed no bacterial deposits on the enamel surface. In samples where Streptococcus mutans attached to enamel, an extracellular, pellicle-like material was associated with the bacteria adjacent to the enamel. This material appeared to bind to the enamel surface and to mediate bacterial attachment. Membrane-filtered (Millipore Corp.) saliva deposited a thin surface layer on the enamel, but there were no observable alterations of S. mutans attachment to enamel pretreated with saliva. It was noted that Bratthall serotype c and e strains of S. mutans, when grown in glucose-containing medium, attached, although less tenaciously, to enamel and nichrome wires. Chemical and gas chromatographic analyses of cell-associated materials formed by serotype c and e strains cultured in glucose-containing medium revealed low amounts of glucose-positive material and no polymer linkages characteristic of glucan; yet the same strains cultured in sucrose-containing medium had relatively high amounts of glucose-positive material, with polymer linkages-characteristic of glucan. Serotype a, b, and d strains could attach only in sucrose-containing media.
机译：体外模型由提取的人类臼齿的牙釉质组成，悬浮在接种的培养管中的电线上，用于研究细菌对牙釉质的粘附。在没有肉眼可见的斑块形成的条件下，电子显微照片显示牙釉质表面没有细菌沉积。在变形链球菌附着在牙釉质上的样品中，细胞外的表膜状材料与邻近牙釉质的细菌有关。该材料似乎结合到搪瓷表面并介导细菌附着。膜过滤（Millipore Corp.）的唾液在牙釉质上沉积了一层薄薄的表面层，但没有观察到变形链球菌对唾液预处理的牙釉质的附着变化。注意到当在含葡萄糖的培养基中生长时，变形链球菌的Bratthall血清型c和e菌株虽然不那么顽强地附着在漆包线和镍铬合金丝上。化学和气相色谱法分析了在含葡萄糖的培养基中培养的血清型c和e菌株形成的细胞相关物质，显示出少量的葡萄糖阳性物质，没有葡聚糖的聚合物键。然而，在含蔗糖的培养基中培养的相同菌株具有相对较高的葡萄糖阳性物质，具有葡聚糖的聚合物键合特性。血清型a，b和d菌株只能在含蔗糖的培养基中附着。

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