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首页> 外文期刊>Infection and immunity >Use of monoclonal antibodies to detect antigens of Toxoplasma gondii in serum and other body fluids.
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Use of monoclonal antibodies to detect antigens of Toxoplasma gondii in serum and other body fluids.

机译：使用单克隆抗体检测血清和其他体液中的弓形虫抗原。

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Monoclonal antibodies to Toxoplasma gondii were used in an enzyme-linked immunosorbent assay to detect antigens of the parasite in toxoplasma lysate, in peritoneal fluid of mice, and in sera from humans acutely infected with T. gondii. Four of the six monoclonal antibodies were able to detect antigens of toxoplasma in these specimens. Control sera from individuals not infected with T. gondii and from individuals chronically infected with the parasite were negative in the enzyme-linked immunosorbent assay. Sera from individuals not infected with T. gondii but with positive titers from rheumatoid factor were also negative; 2 or 10 sera from individuals not infected with T. gondii but with positive titers for antinuclear antibodies reacted with the monoclonal antibodies. When the results of the enzyme-linked immunosorbent assay with monoclonal antibodies and with the F(ab)2 fraction of an immunoglobulin G from a rabbit infected with T. gondii were compared, it was noted that the F(ab)2 was more active in detecting parasite antigens than were the monoclonal antibodies. Thus, although monoclonal antibodies can be used to detect antigens of T. gondii in sera and other body fluids, polyvalent antibody (such as the F(ab)2 fraction) appears to be more satisfactory for this purpose.
机译：弓形虫的单克隆抗体用于酶联免疫吸附测定中，以检测弓形虫溶胞产物，小鼠腹膜液和急性感染弓形虫的人血清中的寄生虫抗原。六个单克隆抗体中的四个能够检测这些标本中的弓形虫抗原。在酶联免疫吸附试验中，未感染弓形虫的个体和长期感染该寄生虫的个体的对照血清均为阴性。未感染刚地弓形虫但风湿因子滴度呈阳性的人的血清也呈阴性。来自未感染弓形虫的人的2或10份血清，但抗核抗体滴度为正，与单克隆抗体反应。当比较用单克隆抗体和弓形虫感染的兔的免疫球蛋白G的F（ab）2分数进行的酶联免疫吸附测定的结果时，注意到F（ab）2的活性更高在检测寄生虫抗原方面要比单克隆抗体高。因此，尽管单克隆抗体可用于检测血清和其他体液中的弓形虫抗原，但多价抗体（例如F（ab）2组分）似乎更令人满意。

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