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首页> 外文期刊>Infection and immunity >Protective Effect of Immunization of Rats with Holotoxin or B Subunit of Escherichia coli Heat-Labile Enterotoxin
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Protective Effect of Immunization of Rats with Holotoxin or B Subunit of Escherichia coli Heat-Labile Enterotoxin

机译：大肠杆菌热不稳定肠毒素的全毒素或B亚基对大鼠免疫的保护作用

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The relative immunogenicities of three forms of the Escherichia coli heatlabile enterotoxin (LT), the holotoxin, its B subunit, and the polymyxin-release form (PM LT) were compared by immunizing rats with various dosages of each given exclusively by the parenteral (IP/IP) or peroral (PO/PO) routes or by a combination of the two (IP/PO). The degree of protection was evaluated by challenge in ligated ileal loops, and the serum antitoxin response was determined by an enzyme-linked immunosorbent assay with homologous antigens. When given by the PO/PO route, each LT antigen provided only weak protection against the toxin and virtually none against viable LT-producing strains; serum antitoxin titers were not significantly increased. When the toxins were given after a parental primary immunization by either the IP/IP or the IP/PO routes, each LT antigen provided a dose-related increase in serum antitoxin titers and in the degree of protection against the toxin as well as against viable strains which produce LT alone (LT+/ST?) or in combination with the heat-stable toxin (LT+/ST+). The degree of protection against viable bacteria, particularly the LT+/ST+ strain, was stronger in animals which received booster immunizations by the PO route. When expressed on the basis of molar equivalents, holotoxin provided significant protection (a protection index of >5 against toxin challenge and >50% reduced secretion with bacterial challenge) with 4 to 15 times fewer moles than PM LT and up to 50 times fewer moles than the B subunit. These observations indicate that, on the basis of molar equivalents, the holotoxin (which contains one A plus five or six B subunits) is a more potent immunogen than either PM LT (which contains one A and probably one B subunit) or the B subunit.
机译：比较三种形式的大肠埃希氏菌>不耐热肠毒素（LT），全毒素，其B亚基和多粘菌素释放形式（PM LT）的相对免疫原性。仅通过肠胃外（IP / IP）或经口（PO / PO）路线或两者（IP / PO）的组合给出。通过在结扎的回肠环中激发来评估保护程度，并且通过具有同源抗原的酶联免疫吸附测定来确定血清抗毒素反应。当通过PO / PO途径给予时，每种LT抗原仅提供针对毒素的弱保护，而实际上不提供针对活的LT产生菌株的保护。血清抗毒素滴度没有明显增加。当通过IP / IP或IP / PO途径在父母进行初次免疫后给予毒素时，每种LT抗原均会引起血清抗毒素效价的剂量相关增加，以及对毒素的抵抗程度以及对存活的抵抗力单独产生LT（LT + / ST ？）或与热稳定毒素结合产生（LT + / ST + ）。在通过PO途径接受加强免疫的动物中，对活菌尤其是LT + / ST + 菌株的保护程度更强。当以摩尔当量表示时，全毒素提供了显着的保护作用（对毒素攻击的保护指数> 5，对细菌攻击的分泌减少> 50％），其摩尔数比PM LT低4至15倍，而摩尔数最多减少50倍比B亚基这些观察结果表明，基于摩尔当量，全毒素（包含一个A加五个或六个B亚基）是比PM LT（包含一个A和一个B可能亚基）或B亚基更强的免疫原。 。

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