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首页> 外文期刊>Infection and immunity >Fibrinogen enhances complement-mediated augmentation of retention of polymorphonuclear leukocytes by nylon columns.
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Fibrinogen enhances complement-mediated augmentation of retention of polymorphonuclear leukocytes by nylon columns.

机译：纤维蛋白原通过尼龙柱增强补体介导的多形核白细胞保留的增加。

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Retention of polymorphonuclear leukocytes (PMN) by nylon columns is significantly increased when PMN are suspended in zymosan-activated plasma. In zymosan-activated plasma, 25.8 +/- 4.9% of PMN were retrained as compared with 11.1 +/- 2.7% suspended in zymosan-activated serum (ZAS) (P = 0.001) and 7.9 +/- 3.2% in fresh serum (P = 0.0005). Addition of heated plasma (56 degrees C for 30 min) to ZAS restored optimal retention. These results suggested a role for a heat-stable factor(s) in plasma, which augmented complement-mediated enhancement of PMN adhesiveness. This study was designed to determine whether fibrinogen enhanced retention by nylon columns of PMN in the presence of activated complement. Addition of defibrinated plasma to ZAS failed to enhance adhesiveness to nylon. The retention of PMN suspended in ZAS was 11.1 +/- 2.7%, as compared with 10.5 +/- 3.7% in ZAS plus defibrinated plasma. Retention of PMN suspended in ZAS plus fibrinogen was 27.92 +/- 13.2% as opposed to 16.35 +/- 7.5% (P = 0.045) in ZAs. In contrast, retention of cells in serum was 12.96 +/- 7.5%. These results suggest that the process which enhances PMN adhesiveness involves the interaction of cells, activated complement, and fibrinogen.
机译：当PMN悬浮在酵母聚糖激活的血浆中时，尼龙柱对多形核白细胞（PMN）的保留显着增加。在酵母聚糖激活的血浆中，重新训练了25.8 +/- 4.9％的PMN，而悬浮在酵母聚糖激活的血清（ZAS）中的11.1 +/- 2.7％（P = 0.001）和新鲜血清中的7.9 +/- 3.2％（ P = 0.0005）。将加热的血浆（56摄氏度，30分钟）添加到ZAS中可恢复最佳保留。这些结果暗示了血浆中热稳定因子的作用，其增加了补体介导的PMN粘附性的增强。这项研究旨在确定在激活补体存在下，纤维蛋白原是否能增强PMN尼龙柱的保留能力。在ZAS中添加去纤维化血浆无法增强对尼龙的粘合性。悬浮在ZAS中的PMN的保留率为11.1 +/- 2.7％，而ZAS加去纤维化血浆中的PMN的保留率为10.5 +/- 3.7％。悬浮在ZAS和纤维蛋白原中的PMN的保留率为27.92 +/- 13.2％，而ZAs中为16.35 +/- 7.5％（P = 0.045）。相反，细胞在血清中的保留为12.96 +/- 7.5％。这些结果表明，增强PMN粘附性的过程涉及细胞，活化的补体和纤维蛋白原的相互作用。

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