• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Effect of formalin toxoiding on Pseudomonas aeruginosa toxin A: biological, chemical, and immunochemical studies.
【24h】

Effect of formalin toxoiding on Pseudomonas aeruginosa toxin A: biological, chemical, and immunochemical studies.

机译：福尔马林类毒素对铜绿假单胞菌毒素A的影响：生物学，化学和免疫化学研究。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We investigated the effect of Formalin toxoiding on the biological, chemical, and immunological activities of Pseudomonas aeruginosa toxin A. Formalin treatment alone resulted in a 1,000-fold decrease in toxin-induced cell cytotoxicity and altered the antigenicity of the toxin A molecule without adversely affecting enzymatic activity. Competitive blocking experiments indicated that Formalin-mediated detoxification proceeded via alterations in a region of the toxin molecule other than the active site of the enzyme. The addition of lysine to Formalin-toxin mixtures not only increased the rate and extent of detoxification and antigenic alteration, but also completely destroyed enzymatic activity. The immunogenicities of different toxoids varied substantially. Upon dialysis and storage, Formalin-derived toxoids underwent partial toxic reversion, whereas a Formalin-lysine-derived toxoid did not. The sodium dodecyl sulfate-polyacrylamide gel patterns of Formalin- and Formalin-lysine-treated toxins indicated that these treatments caused the formation of a heterogenous group of high-molecular-weight species and produced substantial changes in the electrophoretic mobility of toxin A.
机译：我们调查了福尔马林类毒素对铜绿假单胞菌毒素A的生物学，化学和免疫学活性的影响。单独的福尔马林治疗导致毒素诱导的细胞毒性降低了1000倍，并且改变了毒素A分子的抗原性，而没有不利影响酶活性。竞争性阻断实验表明，福尔马林介导的排毒是通过改变毒素分子除酶活性位点以外的区域进行的。在福尔马林毒素混合物中添加赖氨酸不仅增加了解毒和抗原改变的速度和程度，而且还完全破坏了酶的活性。不同类毒素的免疫原性差异很大。透析和储存后，福尔马林衍生的类毒素经历部分毒性逆转，而福尔马林赖氨酸衍生的类毒素则没有。福尔马林和福尔马林-赖氨酸处理过的毒素的十二烷基硫酸钠-聚丙烯酰胺凝胶图谱表明，这些处理导致形成了高分子量物种的异质基团，并且毒素A的电泳迁移率发生了实质性变化。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号