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首页> 外文期刊>Infection and immunity >Relationship of HeLa cell infectivity to biochemical, serological, and virulence characteristics of Yersinia enterocolitica.
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Relationship of HeLa cell infectivity to biochemical, serological, and virulence characteristics of Yersinia enterocolitica.

机译：HeLa细胞感染性与小肠结肠炎耶尔森氏菌的生化，血清学和毒力特征之间的关系。

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Examination of 69 strains of Yersinia enterocolitica which represented 20 serotypes and nontypable isolates for HeLa cell infectivity by a roller tube technique that provided a quantitative index of infection showed that infectivity (index, greater than 3.50) was confined to strains of serotypes O:8, O:3, O:5, 27, O:9, O:1, O:1,2,3, O:2,3, O:4,32, and O:21. All strains that were HeLa positive were sucrose positive and negative for salicin, esculin, rhamnose, raffinose, melibiose, alpha-methylglucoside, and citrate. All HeLa-negative strains were either sucrose and salicin positive or were sucrose negative. Twenty-one strains were examined for virulence by the ability to produce guinea pig conjunctivitis and diarrhea in mice. Positive strains were limited to those that were HeLa positive and were autoagglutination positive and calcium dependent at 35 degrees C. There was no association between virulence and the ability to produce enterotoxin measured by the infant mouse assay. Loss of autoagglutinability and calcium dependency was accompanied by loss of virulence, but HeLa cell infectivity was unchanged. The results suggest that at least two properties are necessary for virulence: the presence of the V and W antigens, mediated by the same plasmid as autoagglutination and calcium dependency, and an invasive factor demonstrated in vitro by HeLa cell infectivity. These virulence properties are found in only certain biotypes of Y. enterocolitica.
机译：通过提供定量感染指数的滚管技术对代表20种血清型和非典型分离株的HeLa细胞感染性的69株耶尔森氏小肠结肠炎耶尔森氏菌进行了检查，结果表明感染性（指数大于3.50）仅限于O：8血清型。 O：3，O：5、27，O：9，O：1，O：1,2,3，O：2,3，O：4,32和O：21。 HeLa阳性的所有菌株对水杨素，七叶树素，鼠李糖，棉子糖，蜜三糖，α-甲基葡糖苷和柠檬酸盐的蔗糖均为阳性，阴性。所有HeLa阴性菌株均为蔗糖和水杨素阳性或蔗糖阴性。通过在小鼠中产生豚鼠结膜炎和腹泻的能力检查了二十一种菌株的毒力。阳性菌株仅限于HeLa阳性，自凝集阳性和在35摄氏度下依赖钙的菌株。通过婴儿小鼠试验测定，毒力与产生肠毒素的能力之间没有关联。自凝性和钙依赖性的丧失伴随着毒性的丧失，但HeLa细胞的感染性没有改变。结果表明，毒力至少需要两个属性：V和W抗原的存在（由与自动凝集和钙依赖性相同的质粒介导），以及在体外通过HeLa细胞感染性证明的侵袭因子。这些毒力特性仅在小肠结肠炎耶尔森菌的某些生物型中发现。

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