Plaque Formation by Rickettsia conori in WI-38, DBS-FRhL-2, L-929, HeLa, and Chicken Embryo Cells

Joseph V. Osterman; Ronald P. Parr

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Plaque Formation by Rickettsia conori in WI-38, DBS-FRhL-2, L-929, HeLa, and Chicken Embryo Cells

机译：立克次体在WI-38，DBS-FRhL-2，L-929，HeLa和鸡胚细胞中形成斑块

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Mammalian cells particularly suitable for the study of specialized aspects of rickettsial biology were tested for their ability to support plaque formation by Rickettsia conori. The detection of plaques was substantially influenced by the combination of growth medium and cell type used. Large plaques (2.0 to 3.0 mm in diameter) occurred by 8 days postinfection in WI-38 and DBS-FRhL-2 cells supported by medium 199. Smaller plaques (0.5 to 1.0 mm in diameter) were seen in L-929 and HeLa cells at 8 to 11 days postinfection and were more discernible in cells supported with Eagle minimal essential medium. Chicken embryo cells maintained in Dulbecco's modified Eagle medium exhibited large spherical plaques with a diameter of approximately 1.5 mm by 8 days postinfection.

机译：通过 Rickettsia conori 测试了特别适合用于立克次体生物学特定方面研究的哺乳动物细胞支持斑块形成的能力。斑块的检测很大程度上受生长培养基和所用细胞类型的组合影响。感染后第8天，在中等培养基199支持的WI-38和DBS-FRhL-2细胞中出现大斑块（直径2.0至3.0 mm）。在L-929和HeLa细胞中观察到较小的斑块（直径0.5至1.0 mm）感染后8到11天，在以Eagle基本必需培养基支持的细胞中更易辨别。感染后8天，在Dulbecco改良的Eagle培养基中保存的鸡胚细胞显示出直径约为1.5 mm的大球形斑块。 展开▼

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《Infection and immunity》 |1974年第5期|共4页

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Joseph V. Osterman; Ronald P. Parr;
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中图分类医学免疫学;

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1. Infection cycle of Rickettsia rickettsii in chicken embryo and L-929 cells in culture. [J] . C L Wisseman, E A Edlinger, A D Waddell, Infection and immunity . 1976,第4期

机译：立克次体立克次体在鸡胚和培养中的L-929细胞中的感染周期。

2. In Vitro Studies on Rickettsia-Host Cell Interactions: Intracellular Growth Cycle of Virulent and Attenuated Rickettsia prowazeki in Chicken Embryo Cells in Slide Chamber Cultures [J] . C. L. Wisseman, A. D. Waddell Infection and immunity . 1975,第6期

机译：体外研究Rickettia-宿主细胞相互作用：毒株毒力和衰减的Rickettsia prowazeki的细胞内生长循环在湿室培养中的鸡胚细胞中

3. In Vitro Studies on Rickettsia-Host Cell Interactions: Intracellular Growth Cycle of Virulent and Attenuated Rickettsia prowazeki in Chicken Embryo Cells in Slide Chamber Cultures [J] . C. L. Wisseman, A. D. Waddell Infection and immunity . 1975,第6期

机译：体外研究Rickettia-宿主细胞相互作用：毒株毒力和衰减的Rickettsia prowazeki的细胞内生长循环在湿室培养中的鸡胚细胞中

4. Micro-Raman spectroscopy study of ALVAC virus infected chicken embryo cells [C] . Anupam K. Misra, Lori E. Kamemoto, Ningjie Hu, Smart biomedical and physiological sensor technology VIII . 2011

机译：ALVAC病毒感染的鸡胚细胞的显微拉曼光谱研究

5. ULTRASTRUCTURAL STUDIES OF THE MORPHOLOGY AND DIVISION OF THE RICKETTSIA-LIKE MICROORGANISM, WOLBACHIA PERSICA IN THE CHICKEN EMBRYO. [D] . MERLINE, JOSEPH RAYMOND. 1972

机译：鸡胚类立克次氏体微生物（Wolbachia Persica）的形态和分区的超微结构研究。

6. Plaque Formation by Rickettsia conori in WI-38 DBS-FRhL-2 L-929 HeLa and Chicken Embryo Cells [O] . Joseph V. Osterman, Ronald P. Parr 1974

机译：立克次体在WI-38DBS-FRhL-2L-929HeLa和鸡胚细胞中形成斑块

7. Plaque Formation by Rickettsia conori in WI-38, DBS-FRhL-2, L-929, HeLa, and Chicken Embryo Cells [O] . Joseph V. Osterman, Ronald P. Parr 1974

机译：Rickettia Conori在Wi-38，DBS-FRHL-2，L-929，Hela和鸡胚细胞中的斑块形成

8. Improved Chicken Embryo Cell Culture Plaque Assay for Scrub Typhus Rickettsiae. [R] . Woodman, D. R., Grays, R., Weiss, E. 1977

机译：改良鸡胚胎细胞培养斑块测定灌木斑疹伤寒立克次体。

1. METHODS FOR SELECTING GERM-LINE COMPETENT CELLS IN CHICKEN EMBRYOS, AND THE USE OF THE CELLS IN THE PRODUCTION OF CHIMERAS [P] . 外国专利： EP0973870A1 . 2000-01-26

机译：鸡胚生殖细胞竞争性细胞的选择方法及其在嵌合体生产中的用途

2. METHODS FOR SELECTING GERM-LINE COMPETENT CELLS IN CHICKEN EMBRYOS, AND THE USE OF THE CELLS IN THE PRODUCTION OF CHIMERAS [P] . 外国专利： CA2282791A1 . 1998-09-03

机译：鸡胚生殖细胞竞争性细胞的选择方法及其在嵌合体生产中的用途

3. METHODS FOR SELECTING GERM-LINE COMPETENT CELLS IN CHICKEN EMBRYOS, AND THE USE OF THE CELLS IN THE PRODUCTION OF CHIMERAS [P] . 外国专利： WO9838283A1 . 1998-09-03

机译：鸡胚生殖细胞竞争性细胞的选择方法及其在嵌合体生产中的用途

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Plaque Formation by Rickettsia conori in WI-38, DBS-FRhL-2, L-929, HeLa, and Chicken Embryo Cells

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