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首页> 外文期刊>Infection and immunity >Interaction of murine cytomegalovirus with separated populations of spleen cells.
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Interaction of murine cytomegalovirus with separated populations of spleen cells.

机译：鼠巨细胞病毒与分离的脾细胞群体的相互作用。

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Mouse spleen cultures were infected with murine cytomegalovirus, either before or after separating the cells into various classes, and the growth of virus and production of infectious centers were measured in each cell class. The separation techniques, which were used in various combinations, comprised: adherence to plastic surfaces; adherence to nylon wool columns; gamma irradiation; and treatment with anti-immunoglobulin serum plus complement. The "macrophage population" took up most of the input virus, as shown by autoradiography of spleen cells infected with radioactive virus. These cells formed infectious centers but replicated the virus only transiently. In contrast, the "B-lymphocyte population" contained a minor cell fraction which was permissive for replication. The "T-lymphocyte population" appeared to be nonpermissive for replication and did not produce infectious centers.
机译：在将小鼠分为不同类别之前或之后，用小鼠巨细胞病毒感染小鼠脾脏培养物，并在每种细胞类别中测量病毒的生长和感染中心的产生。用于各种组合的分离技术包括：粘附到塑料表面上;遵守尼龙羊毛柱;伽马射线并用抗免疫球蛋白血清加补体治疗。放射病毒感染的脾细胞的放射自显影显示“巨噬细胞群”吸收了大部分输入病毒。这些细胞形成感染中心，但仅瞬时复制病毒。相反，“ B淋巴细胞群体”包含允许复制的较小细胞部分。 “ T淋巴细胞群体”似乎不允许复制，并且不会产生感染中心。

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