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首页> 外文期刊>Infection and immunity >Binding of antigen-enzyme complex by antigen-binding cells as an approach for immunogdiagnosis.
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Binding of antigen-enzyme complex by antigen-binding cells as an approach for immunogdiagnosis.

机译:抗原结合细胞与抗原-酶复合物的结合作为免疫诊断的一种方法。

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摘要

An immunoenzyme method capable of detecting specific antigen-binding cells is developed. The method is based on the chemical coupling of the antigen to beta-galactosidase and binding of the conjugate to the corresponding antigen-binding cells. Human hydatid fluid was used as an antigen model, and cells derived from rabbits immunized with human hydatid fluid showed specific binding to human hydatid fluid-beta-galactosidase conjugate. The method is specific and reproducible and could be used as an immunodiagnostic test for various diseases in which little or no antibody is produced.
机译:开发了一种能够检测特异性抗原结合细胞的免疫酶方法。该方法基于抗原与β-半乳糖苷酶的化学偶联以及缀合物与相应抗原结合细胞的结合。将人hy虫液用作抗原模型,并且用人hy虫液免疫的兔来源的细胞显示出与人hy虫液-β-半乳糖苷酶缀合物的特异性结合。该方法是特异性和可重现的,并且可以用作对几乎不产生抗体或不产生抗体的各种疾病的免疫诊断测试。

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