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首页> 外文期刊>Infection and immunity >Fractionation of Schistosoma japonicum soluble egg antigen glycoproteins by hydrophobic interaction chromatography.
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Fractionation of Schistosoma japonicum soluble egg antigen glycoproteins by hydrophobic interaction chromatography.

机译：通过疏水相互作用色谱分离日本血吸虫可溶性蛋抗原糖蛋白。

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We are currently studying the soluble egg antigens of Schistosoma japonicum in an attempt to determine which antigens are potent immunogens. Previously, we demonstrated by Ouchterlony immunodiffusion and inhibition of the circumoval precipitin test that the glycoprotein fraction of soluble egg antigens contains the antigens which are most immunogenic in natural infections. The soluble egg antigen glycoproteins have now been further fractionated via hydrophobic interaction chromatography on phenyl Sepharose. We found that there were at least two antigens involved in the circumoval precipitin reaction. Both the hydrophilic antigen which we call japonicum antigen glycoprotein II (JAG II) and a mixture of hydrophobic antigens (JAG III and the JAG IV complex) were capable of causing a 50% inhibition of the COP reaction around S. japonicum eggs. JAG II was not a major serological antigen of S. japonicum since it gave only a weak precipitin line upon Ouchterlony immunodiffusion analysis with pooled sera from Filipino patients with chronic S. japonicum infections. Hydrophobic interaction chromatography yielded preparations which were sufficiently pure for use in radioimmunoassays. By radioimmunoassay, the best antigens among the glycoproteins were moderately hydrophobic JAG III and the JAG IV complex. They had large amounts of antibody directed toward them in patients with schistosomiasis japonica and exhibited little reactivity with S. mansoni. The hydrophilic glycoproteins JAG I and II were poor immunogens and extensively cross-reacted with S. mansoni. This cross-reactivity means that diagnostic tests with crude soluble egg antigens would run the risk of potential false-negative results in patients with other trematode infections.
机译：我们目前正在研究日本血吸虫的可溶性卵抗原，以试图确定哪些抗原是有效的免疫原。以前，我们通过Ouchterlony免疫扩散和抑制环周沉淀蛋白试验证明，可溶性蛋抗原的糖蛋白部分含有在自然感染中最具免疫原性的抗原。现在已经通过在苯基琼脂糖凝胶上的疏水相互作用色谱法进一步分离了可溶性蛋抗原糖蛋白。我们发现至少有两种抗原参与了环膜沉淀素反应。我们称为日本ap抗原糖蛋白II（JAG II）的亲水性抗原和疏水性抗原的混合物（JAG III和JAG IV复合物）都能够引起日本血吸虫卵周围COP反应的50％抑制。 JAG II不是日本血吸虫的主要血清学抗原，因为根据Ouchterlony免疫扩散分析，菲律宾人患有慢性日本血吸虫感染的患者血清中仅给出了较弱的沉淀素系。疏水相互作用色谱法产生的制剂纯度足以用于放射免疫测定中。通过放射免疫分析，糖蛋白中最好的抗原是中等疏水的JAG III和JAG IV复合物。在日本血吸虫病患者中，它们具有针对他们的大量抗体，并且与曼氏链球菌几乎没有反应。亲水性糖蛋白JAG I和II免疫原性较差，并与曼氏链球菌广泛交叉反应。这种交叉反应性意味着用粗制可溶性卵抗原进行的诊断测试将冒着其他吸虫感染患者潜在假阴性结果的风险。

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