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首页> 外文期刊>Infection and immunity >Opsonization in vitro of Giardia lamblia trophozoites.
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Opsonization in vitro of Giardia lamblia trophozoites.

机译：贾第鞭毛虫滋养体的体外调理作用。

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摘要

The ability of peritoneal rabbit macrophages from immunized and nonimmunized animals to phagocytose Giardia lamblia trophozoites in the presence of serum was studied and compared in an in vitro system. The rabbits which served as the source of immune serum and macrophages were injected repeatedly at multiple sites (intramuscularly, subcutaneously, and intradermally) with a mixture of G. lamblia trophozoites and Freund complete adjuvant. In the presence of normal rabbit serum, a low level of phagocytosis of Giardia trophozoites by normal and immune macrophages was observed. In the presence of hyperimmune rabbit serum, an increased phagocytic activity of both types of macrophages occurred. The opsonic activity was similar whether whole serum or purified immunoglobulin G was used and whether or not these were heat inactivated. G. lamblia trophozoites in suspension were shown to be agglutinated in the presence of hyperimmune serum. Tests employing serial dilutions of hyperimmune serum resulted in a parallel loss of opsonifying and agglutinating activities. It is suggested that opsonization in vivo may play a role in the ability of the host to limit infection by these organisms.
机译：在体外系统中研究并比较了来自免疫动物和非免疫动物腹膜兔巨噬细胞吞噬贾第鞭毛贾第鞭毛虫滋养体的能力。在多个部位（肌肉内，皮下和皮内）反复注射​​作为免疫血清和巨噬细胞来源的兔子，并加入兰伯氏菌滋养体和弗氏完全佐剂的混合物。在正常兔血清存在下，观察到正常和免疫巨噬细胞对贾第鞭毛虫滋养体的吞噬作用较低。在超免疫兔血清的存在下，两种巨噬细胞的吞噬活性均增加。无论使用全血清还是纯化的免疫球蛋白G，以及是否将它们热灭活，其调理活性均相似。悬浮液中的兰氏芽孢杆菌滋养体显示在超免疫血清存在下凝集。使用高免疫血清的系列稀释液进行的测试导致调理和凝集活性平行丧失。提示体内调理作用可能在宿主限制这些生物体感染的能力中起作用。

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