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首页> 外文期刊>Infection and immunity >Production, purification, and characterization of the fimbrial adhesive antigen F41 isolated from calf enteropathogenic Escherichia coli strain B41M.
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Production, purification, and characterization of the fimbrial adhesive antigen F41 isolated from calf enteropathogenic Escherichia coli strain B41M.

机译：从小牛肠道致病性大肠杆菌B41M菌株分离的纤维粘附抗原F41的生产，纯化和鉴定。

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摘要

Enterotoxigenic Escherichia coli strains of serogroups O9 and O101 produce an adhesive antigen, provisionally designated as F41. Production of the F41 antigen was shown to be dependent on the composition of the growth medium. A minimal salts medium or Minca medium was the most suitable medium to obtain a high production. The biosynthesis of the F41 antigen was repressed at 18 degrees C or in the presence of L-alanine. The F41 antigen was isolated from the bacteria by mechanical detachment, concentrated by precipitation with ammonium sulfate, and purified gel filtration on Sepharose CL-4B and treatment with deoxycholate. The purified F41 was composed of protein subunits with an apparent molecular weight of 29,500 on sodium dodecyl sulfate-polyacrylamide gels. The isoelectric point of the antigen was 4.6. The N-terminal amino acid sequence was determined. The F41 antigen had strong hemagglutinating activity with guinea pig and human group A erythrocytes and weaker hemagglutinating activity with horse and sheep erythrocytes. In immunoelectrophoresis at pH 8.4 the purified antigen migrated to the anode. Purified F41 antigen has a filamentous structure with an average diameter of 3.2 nm.
机译：O9和O101血清群的产肠毒素大肠杆菌菌株产生一种粘附抗原，临时命名为F41。已显示F41抗原的产生取决于生长培养基的组成。基本的盐培养基或Minca培养基是获得高产量的最合适培养基。 F41抗原的生物合成在18摄氏度或L-丙氨酸存在下被抑制。通过机械分离从细菌中分离出F41抗原，通过硫酸铵沉淀进行浓缩，并在Sepharose CL-4B上进行纯化的凝胶过滤，并用脱氧胆酸盐处理。纯化的F41由十二烷基硫酸钠-聚丙烯酰胺凝胶上的表观分子量为29,500的蛋白质亚基组成。抗原的等电点为4.6。确定了N末端氨基酸序列。 F41抗原对豚鼠和人A组红细胞具有较强的血凝活性，而对马和绵羊红细胞具有较弱的血凝活性。在pH 8.4的免疫电泳中，纯化的抗原迁移到阳极。纯化的F41抗原具有平均直径为3.2nm的丝状结构。

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