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首页> 外文期刊>Infection and immunity >Inhibition of onset of overt multiplication of Chlamydia psittaci in persistently infected mouse fibroblasts (L cells).
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Inhibition of onset of overt multiplication of Chlamydia psittaci in persistently infected mouse fibroblasts (L cells).

机译：在持续感染的小鼠成纤维细胞（L细胞）中抑制鹦鹉热衣原体明显繁殖的开始。

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When monolayers of mouse fibroblasts (L cells) persistently infected with Chlamydia psittaci (strain 6BC) were dispersed in medium 199 and plated out in new flasks, the monolayers that grew out consisted almost exclusively of inclusion-free host cells that retained full resistance to superinfection with C. psittaci (covert infection). After a delay that was inversely proportional to the initial density of the newly transferred L cell population, the percentage of host cells containing visible chlamydial inclusions increased rapidly (overt infection), and most of the L cells were destroyed by extensive chlamydial multiplication (wipeout), leaving only a few survivors to start new persistently infected monolayers. When persistently infected L cell populations grown in medium 199 were transferred to Eagle minimal essential medium, the onset of overt multiplication was strongly suppressed although covert multiplication of C. psittaci continued unabated, as shown by host cell retention of resistance to superinfection and the prompt resumption of overt multiplication after transfer back into medium 199. The difference(s) between the two media responsible for the different expression of the persistently infected state was not determined. A single dose of 100 U of penicillin G per ml of medium 199 given at the time persistently infected monolayers were divided almost completely suppressed the appearance of visible signs of chlamydial infection for several weeks, although resistance to superinfection was retained at all times. The same amount of penicillin given 7 days after replating did not prevent the occurrence of the first expected wipeout, but there was a long period of inclusion-free L cell growth between the first wipeout and the second. It was concluded that covert multiplication of C. psittaci in persistently infected L cells may continue indefinitely without the appearance of visible signs of infection. The transition between covert and overt chlamydial multiplication appears to be a penicillin-sensitive, multistep process that is regulated, at least in part, by the host cell density and the composition of the growth medium.
机译：当将持续感染鹦鹉热衣原体（菌株6BC）的小鼠成纤维细胞（L细胞）单层分散在199培养基中并铺在新烧瓶中时，长出的单层几乎完全由对完全感染具有完全抵抗力的无内含宿主细胞组成与鹦鹉热衣原体（隐性感染）。在与新转移的L细胞群体的初始密度成反比的延迟后，包含可见衣原体包涵体的宿主细胞百分比迅速增加（明显感染），并且大部分L细胞被广泛的衣原体繁殖破坏（消失） ，仅剩下少数幸存者来启动新的持久感染单层。当将在199培养基中生长的持续感染的L细胞群体转移到Eagle基本必需培养基中时，尽管鹦鹉热衣原体的隐性繁殖一直没有减弱，但强烈抑制了显性繁殖的开始，如宿主细胞对超级感染的抗性保留和迅速恢复所显示。转移回培养基199后的公倍增殖。未确定造成持续感染状态不同表达的两种培养基之间的差异。在持续感染的单层细胞时，每毫升199培养基中每毫升100 U的青霉素G被分开，几乎可以完全抑制衣原体感染的可见迹象，持续了数周，尽管对超级感染的抵抗力一直保持着。接种后第7天给予相同量的青霉素并不能阻止第一次预期的清除，但是在第一次清除和第二次清除之间存在很长的无包涵L细胞生长。结论是，持续感染的L细胞中隐孢子虫隐性繁殖可能会无限期地持续，而不会出现明显的感染迹象。隐性和显性衣原体增殖之间的过渡似乎是对青霉素敏感的多步过程，该过程至少部分受宿主细胞密度和生长培养基组成的调节。

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