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首页> 外文期刊>Infection and immunity >Immunochemical Properties of Glucosyltransferases from Streptococcus mutans
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Immunochemical Properties of Glucosyltransferases from Streptococcus mutans

机译：变形链球菌葡萄糖基转移酶的免疫化学性质

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Antiserum against purified mutansynthetase (EC 2.4.1.?) of Streptococcus mutans 6715 (serotype g), which is responsible for the synthesis of water-insoluble glucan (ISG) in the presence of both sucrose and water-soluble glucan, was prepared. The specificity of the antiserum was tested by using crude enzyme preparations (CEPs) of S. mutans strains of various serotypes. On immunodiffusion, the antiserum cross-reacted with CEPs from strains of serotypes a (HS-6 and AHT), d (OMZ176), and g (OMZ65 and KIR), but not with those from strains of serotypes b (BHT and FA-1) and c (GS-5 and Ingbritt). The antiserum inhibited the synthesis of ISG by crude or purified mutansynthetase of S. mutans 6715. The activities of ISG synthesis by CEPs from the strains antigenically related in the foregoing immunodiffusion were inhibited by the antiserum against strain 6715 mutansynthetase. The antiserum, however, also inhibited the enzyme activity of the strains of serotype b. The finding that the antiserum against purified dextransucrase of S. mutans HS-6 inhibited ISG synthesis by a CEP of strain HS-6 and also by CEPs of antigenically related strains suggested that dextransucrase activity is involved in ISG synthesis.
机译：抗变形链球菌6715（血清型 g ）的纯净突变酶（EC 2.4.1。？）的抗血清，该酶负责合成水不溶性葡聚糖（ISG）。制备了蔗糖和水溶性葡聚糖的存在。通过使用 S的粗酶制剂（CEP）来测试抗血清的特异性。各种血清型的变异株。在免疫扩散中，抗血清与血清型 a （HS-6和AHT）， d （OMZ176）和 g （OMZ65和KIR），但不包括血清型 b （BHT和FA-1）和 c （GS-5和Ingbritt）的菌株。该抗血清通过 S的粗制或纯化的突变酶抑制ISG的合成。变异体6715。针对上述免疫扩散的抗原相关菌株的CEP合成ISG的活性受到抗变异体6715菌株的抗血清的抑制。然而，抗血清也抑制了血清型 b的菌株的酶活性。发现该抗血清抗 S的纯化葡聚糖酶。突变体HS-6通过HS-6菌株的CEP以及抗原相关菌株的CEP抑制ISG合成，这表明葡聚糖酶活性与ISG合成有关。

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