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首页> 外文期刊>Infection and immunity >Specific absence of type 2 fimbriae on a coaggregation-defective mutant of Actinomyces viscosus T14V.
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Specific absence of type 2 fimbriae on a coaggregation-defective mutant of Actinomyces viscosus T14V.

机译：在粘性放线菌T14V的共聚集缺陷型突变体上没有特定的2型菌毛。

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The coaggregation-defective (COG-) mutant Actinomyces viscosus T14V(PK455), which is unable to participate in lactose-sensitive adherence, and its COG+ parent were compared to structurally define the mutational loss of cell-associated lectin activity. Immunoelectrophoretic comparisons of crude extracts or isolated fimbriae from both strains demonstrated type 2 fimbriae (previously designated Ag2) in preparations obtained from the parent but none in those obtained from the mutant. This result was verified by the immunoelectron-microscopic identification of type 1 (previously designated Ag1 or VAl) and type 2 fimbriae on the parent organism but only type 1 fimbriae on the mutant. A comparison of the amounts of extractable fimbriae of each type and the capacity of the cells to bind 14C-labeled monoclonal antibodies specific for each fimbrial component showed that the mutational loss of type 2 fimbriae had no significant quantitative effect on fimbriation of the COG- mutant with type 1 structures. Cells of A. viscosus T14AV, a mutant with various adherence defects that include the COG- phenotype, displayed fimbriae of both types, but in greatly reduced amounts. Thus, the properties of mutant strain T14V(PK455) associated the lectin activity with type 2 fimbriae, whereas those of strain T14AV provided little insight into the mechanism of lactose-sensitive adherence. In addition, the precise nature of the cell surface modification displayed by strain T14V(PK455) provides clear evidence for the existence of distinct and independent fimbriae on A. viscosus T14V.
机译：不能参与乳糖敏感性粘附的共缺陷缺陷型（COG-）突变型粘性放线菌T14V（PK455）及其COG +亲本在结构上定义了细胞相关凝集素活性的突变损失。两种菌株的粗提物或分离菌毛的免疫电泳比较表明，从亲本获得的制剂中存在2型菌毛（以前称为Ag2），而从突变体获得的制剂中则没有。通过免疫电子显微镜鉴定亲本生物体上的1型（以前称为Ag1或VAl）和2型菌毛，而突变体上仅1型菌毛验证了这一结果。比较每种类型的可提取菌毛的量和细胞结合针对每种纤维组分特异的14C标记的单克隆抗体的能力，表明2型菌毛的突变损失对COG突变体的纤维化没有明显的定量影响1型结构。具有各种粘附缺陷（包括COG表型）的粘液曲霉T14AV的细胞均显示两种类型的菌毛，但数量却大大减少。因此，突变菌株T14V（PK455）的特性将凝集素活性与2型菌毛相关联，而菌株T14AV的特性几乎没有了解乳糖敏感粘附的机制。另外，由菌株T14V（PK455）显示的细胞表面修饰的精确性质提供了明确的证据，证明在粘滞曲霉T14V上存在明显和独立的菌毛。

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