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首页> 外文期刊>Infection and immunity >Studies of leukotoxin from Actinobacillus actinomycetemcomitans using the promyelocytic HL-60 cell line.
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Studies of leukotoxin from Actinobacillus actinomycetemcomitans using the promyelocytic HL-60 cell line.

机译：使用早幼粒细胞HL-60细胞系对放线放线杆菌的白细胞毒素的研究。

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The promyelocytic HL-60 cell line was examined for susceptibility to leukotoxin from Actinobacillus actinomycetemcomitans. Strains of A. actinomycetemcomitans which caused lysis of human peripheral blood polymorphonuclear leukocytes also lysed HL-60 cells as determined by release of intracellular lactate dehydrogenase. The killing of HL-60 cells by A. actinomycetemcomitans was dose dependent and temperature dependent, reached maximal levels after 45 min of incubation, and was inhibited by rabbit antisera to A. actinomycetemcomitans. Of 100 oral isolates of A. actinomycetemcomitans from 55 subjects, 16% from 11 healthy subjects, 43% from 13 adult periodontitis patients, 75% from 4 insulin-dependent diabetics, 66% from 2 generalized juvenile periodontitis patients, and 55% from 25 localized juvenile periodontitis patients produced leukotoxin. The same subject could harbor both leukotoxin-producing and -nonproducing isolates. The significantly higher proportion of leukotoxin-producing isolates in the disease groups compared with the healthy group is consistent with the hypothesis that leukotoxin from A. actinomycetemcomitans is an important virulence factor in the pathogenesis of certain forms of periodontal disease.
机译：检查了早幼粒细胞HL-60细胞系对放线放线杆菌的白细胞毒素的敏感性。通过释放细胞内乳酸脱氢酶确定，引起人外周血多形核白细胞溶解的放线放线杆菌菌株也裂解HL-60细胞。放线杆菌对HL-60细胞的杀伤是剂量依赖性和温度依赖性的，孵育45分钟后达到最高水平，并被兔抗放线杆菌的抗血清抑制。在来自55位受试者的100株放线放线杆菌的口腔分离物中，来自11位健康受试者的16％，来自13位成人牙周炎患者的43％，来自4位胰岛素依赖型糖尿病患者的75％，来自2位一般性青少年牙周炎患者的66％，以及来自25位的25％局部性青少年牙周炎患者产生白细胞毒素。同一主题可能同时包含产生白细胞毒素的菌株和不产生白细胞毒素的菌株。与健康组相比，疾病组中产生白细胞毒素的分离株的比例明显更高，这与假单胞菌的白细胞毒素是某些形式的牙周疾病的发病机理中的重要毒力因子这一假设相一致。

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