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首页> 外文期刊>Infection and immunity >Rabbit antibodies to the cell wall polysaccharide of Streptococcus pneumoniae fail to protect mice from lethal challenge with encapsulated pneumococci.
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Rabbit antibodies to the cell wall polysaccharide of Streptococcus pneumoniae fail to protect mice from lethal challenge with encapsulated pneumococci.

机译：兔抗肺炎链球菌细胞壁多糖的抗体无法保护小鼠免受封装的肺炎球菌致死性攻击。

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摘要

A conjugate, composed of the cell wall polysaccharide (C polysaccharide) of Streptococcus pneumoniae and bovine serum albumin (BSA), was prepared with the bifunctional agent N-succinimidyl-3-(2-pyridyldithio)-propionate. Analysis with monoclonal antibodies provided evidence that the phosphocholine (PC) moiety of the C polysaccharide was retained during the conjugation procedure. The C polysaccharide-BSA conjugate elicited antibodies to C polysaccharide in rabbits; no PC-specific antibodies were detected in globulins prepared from these hyperimmune sera obtained early and late after a second immunization. Rabbit hyperimmune sera were taken after multiple intravenous injections of the pneumococcus strain SRC-2, which has a capsulelike structure composed of the C polysaccharide. Globulin prepared from these antisera had both C polysaccharide- and PC-specific antibodies. Antibodies to C polysaccharide elicited by the C polysaccharide-BSA conjugate failed to protect mice against intraperitoneal challenge with a strain of type 3 or type 6A pneumococci. The anti-SRC-2 globulin conferred protection against both of these pneumococcal strains. Absorption of the SRC-2 globulin with C polysaccharide, however, failed to change its protective activity. These data provide evidence that antibodies to the C polysaccharide do not confer immunity against infection of mice with encapsulated pneumococci inoculated by the intraperitoneal route.
机译：用双功能试剂N-琥珀酰亚胺基-3-（2-吡啶基二硫代）-丙酸酯制备由肺炎链球菌的细胞壁多糖（C多糖）和牛血清白蛋白（BSA）组成的结合物。用单克隆抗体进行分析提供了证据，表明在缀合过程中保留了C多糖的磷酸胆碱（PC）部分。 C多糖-BSA缀合物诱导兔抗C多糖的抗体。在第二次免疫后早期和晚期从这些超免疫血清制备的球蛋白中未检测到PC特异性抗体。多次静脉注射肺炎球菌菌株SRC-2后，获得兔超免疫血清，该菌株的囊状结构由C多糖组成。由这些抗血清制备的球蛋白同时具有C多糖和PC特异性抗体。 C多糖-BSA共轭物引发的C多糖抗体未能保护小鼠免受3型或6A型肺炎球菌菌株的腹膜内攻击。抗SRC-2球蛋白赋予针对这两种肺炎球菌菌株的保护。然而，C多糖对SRC-2球蛋白的吸收未能改变其保护活性。这些数据提供了证据表明，针对C多糖的抗体不能赋予抵抗通过腹膜内途径接种的封装肺炎球菌感染小鼠的免疫力。

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