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首页> 外文期刊>Infection and immunity >Specific attachment of Staphylococcus aureus to immobilized fibronectin.
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Specific attachment of Staphylococcus aureus to immobilized fibronectin.

机译：金黄色葡萄球菌与固定的纤连蛋白的特异性结合。

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Staphylococcus aureus cells have been shown to possess surface-associated proteins with affinity for soluble fibronectin. We have investigated the ability of these surface proteins to mediate attachment to immobilized fibronectin and collagen. Attachment was quantified by determination of bacterial ATP in a bioluminescence assay. The ability to attach to fibronectin- or collagen-coated plastic surfaces was investigated for four S. aureus strains: Cowan 1, Newman, SA113(83A), and Wood 46. Cells from the different strains varied in their attachment properties, but all cells except those of strain Wood 46 attached readily to substrates coated with fibronectin. Only cells from strain Cowan 1 attached reproducibly to collagen-coated substrates in the absence of fibronectin. The attachment of cells from strain SA113(83A) to fibronectin-coated surfaces was shown to be dependent on time, fibronectin concentration, and bacterial growth phase. Soluble fibronectin or NH2-terminal fibronectin fragment (Mr, 29,000) disturbed the attachment to surfaces coated with fibronectin bound to denatured collagen type I. The attachment process to such substrates was also effectively inhibited by preincubating the substrate with fibronectin-binding proteins isolated from S. aureus Newman and SA113 (83A) and purified with affinity chromatography.
机译：金黄色葡萄球菌细胞已显示具有与表面相关的蛋白质，对可溶性纤连蛋白具有亲和力。我们已经研究了这些表面蛋白介导固定的纤连蛋白和胶原蛋白附着的能力。通过在生物发光测定中确定细菌ATP来量化附着。对于四种金黄色葡萄球菌菌株：Cowan 1，Newman，SA113（83A）和Wood 46，研究了其附着在纤连蛋白或胶原涂层塑料表面上的能力。不同菌株的细胞的附着特性不同，但所有细胞除了Wood 46菌株外，它们很容易附着在用纤连蛋白包被的基质上。在不存在纤连蛋白的情况下，只有来自Cowan 1菌株的细胞可复制地附着在胶原蛋白包被的底物上。结果表明，菌株SA113（83A）中的细胞与纤连蛋白包被的表面的附着取决于时间，纤连蛋白的浓度和细菌的生长阶段。可溶性纤连蛋白或NH2末端纤连蛋白片段（Mr，29,000）干扰了附着于变性I型胶原的纤连蛋白包被的表面的附着。通过将底物与从S中分离的纤连蛋白结合蛋白进行预孵育，也可以有效抑制与此类底物的附着过程。用Aureus Newman和SA113（83A）纯化，并用亲和色谱法纯化。

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