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首页> 外文期刊>Infection and immunity >Comparative immunogenicities of Vi polysaccharide-protein conjugates composed of cholera toxin or its B subunit as a carrier bound to high- or lower-molecular-weight Vi.
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Comparative immunogenicities of Vi polysaccharide-protein conjugates composed of cholera toxin or its B subunit as a carrier bound to high- or lower-molecular-weight Vi.

机译：由霍乱毒素或其B亚基作为载体与高分子量或低分子量Vi结合的Vi多糖-蛋白质缀合物的比较免疫原性。

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The effect of molecular weight or size of the components on the immunogenicity of polysaccharide-protein conjugates prepared with the native Vi capsular polysaccharide (Vi) (approximately 3 x 10(3) kilodaltons) or lower-molecular-weight Vi (Vis; approximately 46 kilodaltons) abound to cholera toxin (CT) or to its B subunit (CTB) was studied. In mice, Vi-CT, Vi-CTB, and Vis-CTB elicited higher Vi antibody levels than the Vi alone (P less than 0.0001). Vi-CT and Vi-CTB were more immunogenic than Vis-CTB (P less than 0.01). CT or Vi-CT elicited higher levels of CT antibodies, as measured by enzyme-linked immunosorbent assay, than did CTB or Vi-CTB. In rhesus monkeys, the Vi conjugates elicited higher Vi antibody levels than the Vi alone (P less than 0.01). Vi-CTB elicited higher levels of Vi antibody after each injection than did Vis-CTB. Similar levels of CT antibodies, as measured by enzyme-linked immunosorbent assay, were elicited by all three conjugates. In contrast, Vi-CT elicited higher levels of neutralizing antibodies than Vi-CTB or Vis-CTB when either CT or the related heat-labile toxin of Escherichia coli was used as the antigen. These results indicate that the holotoxin and the native Vi provide the most immunogenic components for conjugates designed to induce both Vi and CT antibodies.
机译：分子量或组分大小对用天然Vi荚膜多糖（Vi）（约3 x 10（3）千道尔顿）或低分子量Vi（Vis;约46）制备的多糖-蛋白缀合物的免疫原性的影响霍乱毒素（CT）或其B亚基（CTB）含量很高。在小鼠中，Vi-CT，Vi-CTB和Vis-CTB引起的Vi抗体水平高于单独的Vi（P小于0.0001）。 Vi-CT和Vi-CTB的免疫原性高于Vis-CTB（P小于0.01）。通过酶联免疫吸附测定，CT或Vi-CT引发的CT抗体水平高于CTB或Vi-CTB。在恒河猴中，Vi缀合物引起的Vi抗体水平高于单独的Vi（P小于0.01）。每次注射后，Vi-CTB引起的Vi抗体水平要高于Vis-CTB。通过所有三种缀合物引发的酶联免疫吸附测定法测定的CT抗体水平相似。相反，当将CT或相关的大肠杆菌热不稳定毒素用作抗原时，Vi-CT引起的中和抗体水平高于Vi-CTB或Vis-CTB。这些结果表明，全毒素和天然Vi为设计用来诱导Vi和CT抗体的结合物提供了最具免疫原性的成分。

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