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首页> 外文期刊>Infection and immunity >Antigenic and genetic relatedness of eight Rickettsia tsutsugamushi antigens.
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Antigenic and genetic relatedness of eight Rickettsia tsutsugamushi antigens.

机译：八个立克次氏Ri虫抗原的抗原性和遗传相关性。

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The genetic and antigenic relatedness of eight antigens in three strains of Rickettsia tsutsugamushi has been studied by using recombinant organisms expressing epitopes of the 150-, 110-, 72-, 58-, 56-, 49-, 47-, and 20-kilodalton (kDa) polypeptide antigens of the Karp strain. Southern blot analysis of Karp, Kato, and Gilliam strain genomic DNA by using probes specific for each antigen class indicated that while strong homology exists between each of the corresponding antigen genes in these three strains, some restriction fragment length polymorphism exists. Antibodies affinity purified against each recombinant antigen class reacted with a comparably sized polypeptide in the Karp, Kato, and Gilliam strains in Western blots (immunoblots). Against more recent human isolates of R. tsutsugamushi, the affinity-purified antibodies against the 58-kDa recombinant antigen (anti-58-kDa) reacted with all nine isolates, anti-56-kDa reacted with eight of nine isolates, anti-47-kDa reacted with eight of nine isolates, anti-72-kDa reacted with eight of nine isolates, and anti-110-kDa reacted with four of nine isolates. Additional analysis indicated that the 110-kDa antigen may contain strain-specific epitopes similar to those previously reported for the 56-kDa polypeptide. Evidently, the strain heterogeneity among scrub typhus rickettsiae is a result of multiple components that exhibit variability in a background of strong homology.
机译：通过使用表达150、110、72、58、56、49、47和20千达尔顿抗原决定簇的重组生物，研究了三种three虫立克次氏菌菌株中8种抗原的遗传和抗原相关性。 Karp株的（kDa）多肽抗原。通过使用针对每种抗原类别的特异性探针对Karp，Kato和Gilliam菌株基因组DNA的Southern印迹分析表明，尽管这三种菌株中每个相应抗原基因之间都存在很强的同源性，但存在一些限制性片段长度多态性。在蛋白质印迹法（免疫印迹）中，针对每种重组抗原类别纯化的抗体亲和力与Karp，Kato和Gilliam菌株中大小相对应的多肽反应。针对最近的human虫分离株，针对58-kDa重组抗原（抗58-kDa）的亲和纯化抗体与所有9种分离株反应，抗56-kDa与9种分离株中的8种反应，抗47 -kDa与9个分离株中的8个反应，抗72-kDa与9个分离株中的8个反应，而抗110-kDa与9个分离株中的4个反应。进一步的分析表明，110-kDa抗原可能含有与先前报道的56-kDa多肽相似的菌株特异性表位。显然，灌木斑疹伤寒立克次体之间的菌株异质性是在强同源性背景下表现出可变性的多种成分的结果。

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