• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Expression of the Bacillus anthracis protective antigen gene by baculovirus and vaccinia virus recombinants.
【24h】

Expression of the Bacillus anthracis protective antigen gene by baculovirus and vaccinia virus recombinants.

机译：杆状病毒和牛痘病毒重组体表达炭疽芽孢杆菌保护性抗原基因。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The gene encoding Bacillus anthracis protective antigen (PA) was modified by site-directed mutagenesis, subcloned into baculovirus and vaccinia virus plasmid transfer vectors (pAcYM1 and pSC-11, respectively), and inserted via homologous recombinations into baculovirus Autographa californica nuclear polyhedrosis virus or vaccinia virus (strains WR and Connaught). Expression of PA was detected in both systems by immunofluorescence assays with antisera from rabbits immunized with B. anthracis PA. Western blot (immunoblot) analysis showed that the expressed product of both systems was slightly larger (86 kilodaltons) than B. anthracis-produced PA (83.5 kilodaltons). Analysis of trypsin digests of virus-expressed and authentic PA suggested that the size difference was due to the presence of a signal sequence remaining with the virus-expressed protein. Immunization of mice with either recombinant baculovirus-infected Spodoptera frugiperda cells or with vaccinia virus recombinants elicited a high-titer, anti-PA antibody response.
机译：通过定点诱变修饰编码炭疽芽孢杆菌保护性抗原（PA）的基因，将其亚克隆到杆状病毒和牛痘病毒质粒转移载体（分别为pAcYM1和pSC-11）中，并通过同源重组插入杆状病毒加州产线虫核多角体病毒或牛痘病毒（WR和Connaught株）。通过免疫荧光测定法用来自用炭疽芽孢杆菌PA免疫的兔的抗血清进行免疫荧光测定，检测了两个系统中PA的表达。 Western blot（免疫印迹）分析显示，两个系统的表达产物（86道尔顿）比炭疽芽孢杆菌生产的PA（83.5道尔顿）稍大。对表达有病毒的和真实的PA的胰蛋白酶消化物的分析表明，大小差异是由于存在与表达病毒的蛋白有关的信号序列。用重组杆状病毒感染的贪夜蛾（Spodoptera frugiperda）细胞或牛痘病毒重组体对小鼠进行免疫，可引起高滴度的抗PA抗体应答。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号