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首页> 外文期刊>Infection and immunity >Characterization of Leishmania major antigen-liposomes that protect BALB/c mice against cutaneous leishmaniasis.
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Characterization of Leishmania major antigen-liposomes that protect BALB/c mice against cutaneous leishmaniasis.

机译：保护BALB / c小鼠免于皮肤利什曼病的利什曼原虫主要抗原脂质体的表征。

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Leishmania major antigen-liposomes prepared as dehydration-rehydration vesicles (DRV) and composed of equimolar amounts of L-alpha-distearoyl phosphatidylcholine and cholesterol confer high-level host-protective immunity against virulent homologous challenge to susceptible BALB/c mice. Physical and antigenic characterization of these protective liposomes is described. Both empty and L. major antigen-DRV were multilamellate and heterogeneous in size, ranging from 0.10 to 2.00 microns. Although the liposomes were made by using a crude mixture of promastigote antigens, lipophosphoglycan covered the liposome surface; this was demonstrated by immunogold electron microscopy. Application of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot (immunoblot) analysis revealed preferential entrapment of the 63-kilodalton promastigote protease (gp63) into the DRV. We suggest that our L. major antigen-DRV merit further study because of their preferential entrapment of these two host protective antigens together with their long in vivo half-life. In addition, this report illustrates that intravenous or subcutaneous immunization of BALB/c mice with the same limited subset of protective antigens, predominantly lipophosphoglycan and gp63, within DRV liposomes leads to either protection and low splenic interleukin-3 production or to nonprotection and high splenic interleukin-3 production, respectively. This was consistent with our hypothesis that differential antigen presentation after administration of the same immunogen by the intravenous or the subcutaneous route results in differential T-cell activation.
机译：利什曼原虫主要抗原脂质体制备为脱水-脱水小泡（DRV），由等摩尔量的L-α-二硬脂酰磷脂酰胆碱和胆固醇组成，可对易感BALB / c小鼠产生强力的宿主保护性免疫力，以抵抗强力同源性攻击。描述了这些保护性脂质体的物理和抗原表征。空的和主要的L.主要抗原-DRV都是多层的，大小不一，范围从0.10至2.00微米。尽管脂质体是通过使用前鞭毛体抗原的粗制混合物制备的，但脂质磷酸聚糖覆盖了脂质体的表面。免疫金电子显微镜证实了这一点。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳的应用和蛋白质印迹（免疫印迹）分析表明，优先将63-千达尔顿前鞭毛体蛋白酶（gp63）捕获到DRV中。我们建议我们主要的L.主要抗原-DRV值得进一步研究，因为它们优先捕获了这两种宿主保护性抗原，并具有较长的体内半衰期。此外，该报告还说明，在DRV脂质体内，以有限的亚保护抗原（主要是脂质磷酸聚糖和gp63）的相同有限子集对BALB / c小鼠进行静脉内或皮下免疫会导致保护和脾脏白细胞介素3的生成减少，或者导致无保护性和脾脏高保护性白细胞介素3的产生。这与我们的假设一致，即通过静脉内或皮下途径施用相同免疫原后差异抗原呈递会导致差异性T细胞活化。

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