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首页> 外文期刊>Infection and immunity >Analysis of adherence of Streptococcus defectivus and endocarditis-associated streptococci to extracellular matrix.
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Analysis of adherence of Streptococcus defectivus and endocarditis-associated streptococci to extracellular matrix.

机译：缺陷链球菌和心内膜炎相关链球菌对细胞外基质的粘附性分析。

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Pathogenesis of nutritionally variant streptococcal (NVS) endocarditis initiates with bacterial attachment to and colonization of the damaged heart valve surface. Underlying extracellular matrix (ECM) exposed to the environment during damage to cardiac endothelium provides additional receptors that could be involved in bacterial adherence. The ability of NVS and endocarditis-associated streptococci to bind ECM was investigated by using an enzyme-linked immunosorbent assay system that incorporated ECM secreted by baby hamster kidney and human umbilical vein endothelial cells in culture. Streptococcus defectivus, the major species isolated from NVS endocarditis cases, bound ECM of fibroblasts and endothelial cells, indicating that the ECM molecule involved in the binding was a common constituent of diverse matrices. The specific binding of S. defectivus to ECM was demonstrated by saturation binding and specific antibody inhibition studies. Of the 15 S. defectivus strains analyzed, 13 bound ECM, whereas Streptococcus adjacens and NVS serotype III strains were unable to bind the matrix. This selective binding suggested that S. defectivus binds to heart valves through a mechanism different from those of other NVS in subacute bacterial endocarditis. A survey of non-NVS streptococcal endocarditis isolates demonstrated that S. mutans, S. mitis, S. sanguis, and S. faecalis also bound ECM, whereas other viridans species were unable to bind the matrix.
机译：营养变异型链球菌（NVS）心内膜炎的发病机理始于细菌附着在受损的心脏瓣膜表面并在其上定植。在心脏内皮细胞受损期间暴露于环境的底层细胞外基质（ECM）提供了可能与细菌粘附有关的其他受体。通过使用酶联免疫吸附测定系统研究了NVS和心内膜炎相关链球菌结合ECM的能力，该系统结合了由仓鼠肾和人脐静脉内皮细胞分泌的ECM进行培养。缺陷链球菌是从NVS心内膜炎病例中分离出的主要物种，结合了成纤维细胞和内皮细胞的ECM，表明参与结合的ECM分子是多种基质的共同组成部分。通过饱和结合和特异性抗体抑制研究证明了缺陷链球菌对ECM的特异性结合。在分析的15个缺陷链球菌菌株中，有13个结合了ECM，而邻链球菌和NVS血清型III菌株则无法结合基质。这种选择性结合表明缺陷性链球菌通过与亚急性细菌性心内膜炎中其他NVS不同的机制与心脏瓣膜结合。对非NVS链球菌性心内膜炎分离株的一项调查表明，变形链球菌，米氏链球菌，桑氏链球菌和粪链球菌也能结合ECM，而其他绿藻类却无法结合基质。

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