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首页> 外文期刊>Infection and immunity >Stage-specific expression and intracellular shedding of the cell surface trans-sialidase of Trypanosoma cruzi.
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Stage-specific expression and intracellular shedding of the cell surface trans-sialidase of Trypanosoma cruzi.

机译：克鲁氏锥虫细胞表面转唾液酸酶的阶段特异性表达和细胞内脱落。

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摘要

We have used antibodies to the Trypanosoma cruzi trans-sialidase and to its product, the host cell invasion-related Ssp-3 epitope, to study the expression of the corresponding antigens during the intracellular development of the parasite and in the extracellular trypomastigotes. As soon as 2 h after host cell invasion, trans-sialidase was no longer detected, whereas the Ssp-3 epitope was still present on intracellular parasites. The amastigotes which subsequently developed remained nonreactive with the antibodies. Expression of enzymatically active T. cruzi trans-sialidase started again only after transformation of the amastigotes into trypomastigotes 72 h after host cell invasion. trans-Sialidase was shed from the trypanosomes into the host cell cytoplasm, where the enzyme accumulated until release of the parasites. All released trypomastigotes expressed trans-sialidase on their surfaces and in the flagellar pockets, but stumpy trypomastigotes were stained more intensely than slender trypomastigotes. Ssp-3, the sialylated reaction product of trans-sialidase, was assembled only after rupture of the host cell membrane and was detected on the plasma membranes and in the flagellar pockets of all trypomastigotes.
机译：我们已经使用了克氏锥虫转唾液酸酶及其产物，即与宿主细胞入侵相关的Ssp-3表位的抗体，来研究寄生虫的胞内发育过程中和胞外锥鞭毛体中相应抗原的表达。宿主细胞侵袭后2小时，不再检测到反唾液酸酶，而Ssp-3表位仍存在于细胞内寄生虫上。随后形成的变形虫仍与抗体不反应。仅在宿主细胞侵袭72小时后，将变形虫转化为锥虫病后，才再次开始表达具有酶活性的克鲁维酵母转唾液酸酶。反式唾液酸酶从锥虫中脱落到宿主细胞的胞质中，该酶在那里积累直至寄生虫释放。所有释放的锥虫都在其表面和鞭毛袋中表达了转唾液酸酶，但与细长锥虫相比，树状锥虫的染色更强烈。 Ssp-3是唾液酸酶的唾液酸化反应产物，仅在宿主细胞膜破裂后才组装，并在质膜和所有锥虫的鞭毛袋中被检测到。

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