...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Construction of a recombinant oral vaccine against Salmonella typhi and Salmonella typhimurium.
【24h】

Construction of a recombinant oral vaccine against Salmonella typhi and Salmonella typhimurium.

机译：抗鼠伤寒沙门氏菌和鼠伤寒沙门氏菌的重组口服疫苗的构建。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The viaB gene coding for the Vi antigen of Salmonella typhi Ty2 was subcloned into expression vector pYA248. The recombinant plasmid was termed SMM202 and transformed into Salmonella typhimurium chi 4072, an attenuated delta cya delta crp mutant. Recombinant S. typimurium Vi4072 had the ability to produce Vi capsular polysaccharide and also to invade and colonize the small intestine, mesenteric lymph nodes, and spleen of BALB/c mice. Mice orally immunized with Vi4072 developed serum and secretory antibody responses to the Vi antigen, as measured by a passive hemagglutination assay. Mice developed a delayed-type hypersensitivity following a footpad injection with Vi antigen after being sensitized orally with a suitable dose of Vi4072. Immunization of mice with Vi4072 afforded complete protection against fatal infection with virulent S. typhi Ty2. All data indicate that this route of antigen delivery is effective for stimulating antibody-mediated immunity and for inducing a cell-mediated immune response in BALB/c mice. Thus, S. typhimurium Vi4072 may serve as a vaccine for protection against typhoid fever and salmonellosis caused by S. typhimurium.
机译：将编码伤寒沙门氏菌Ty2 Vi抗原的viaB基因亚克隆到表达载体pYA248中。将该重组质粒命名为SMM202，并将其转化为鼠伤寒沙门氏菌chi 4072，一种减毒的delta cya delta crp突变体。重组鼠伤寒沙门氏菌Vi4072具有产生荚膜荚膜多糖的能力，并且能够侵袭和定殖BALB / c小鼠的小肠，肠系膜淋巴结和脾脏。用被动式血凝试验测定，用Vi4072口服免疫的小鼠产生了对Vi抗原的血清和分泌抗体应答。在用适当剂量的Vi4072口服敏化后，在足垫注射Vi抗原后，小鼠出现迟发型超敏反应。用Vi4072免疫小鼠可提供针对致命性伤寒链球菌Ty2致命感染的完全保护。所有数据表明该抗原递送途径对于刺激抗体介导的免疫和诱导BALB / c小鼠中细胞介导的免疫应答是有效的。因此，鼠伤寒沙门氏菌Vi4072可用作预防由鼠伤寒沙门氏菌引起的伤寒和沙门氏菌病的疫苗。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号