Binding studies of a monoclonal antibody specific for 3-deoxy-D-manno-octulosonic acid with a panel of Klebsiella pneumoniae lipopolysaccharides representing all of the O serotypes.

N M van der Meer; B J Appelmelk; A M Verweij-van Vught; W Nimmich; P Kosma; L G Thijs; J de Graaff; D M MacLaren

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Binding studies of a monoclonal antibody specific for 3-deoxy-D-manno-octulosonic acid with a panel of Klebsiella pneumoniae lipopolysaccharides representing all of the O serotypes.

机译：对3-脱氧-D-甘露糖辛酸具有特异性的单克隆抗体与代表所有O型血清型的肺炎克雷伯氏菌脂多糖的结合研究。

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A monoclonal antibody (MAb) raised against Salmonella minnesota R595 and specific for alpha-3-deoxy-D-manno-octulosonic acid (alpha-Kdo) of the inner core was tested for binding to lipopolysaccharides (LPS) of Klebsiella pneumoniae. The MAb was tested in several assay systems (enzyme-linked immunosorbent assay, passive hemolysis, and inhibition of passive hemolysis) with a large panel (n = 23) of K. pneumoniae LPS representing all nine currently known O serotypes. MAb 20 showed reactivity with almost all O serotypes of K. pneumoniae LPS, and this reactivity could be inhibited by synthetic Kdo. This suggests an epitope in the cores of these Klebsiella LPS much like that in the inner core of LPS of S. minnesota. Large differences in reactivity between LPS of different strains belonging to the same O serotype were observed. After sodium dodecyl sulfate-polyacrylamide gel electrophoresis of LPS followed by immunoblotting, reactivity of MAb 20 was observed only with the fast-moving fraction possibly representing the nonsubstituted core. No binding was seen with the high-molecular-weight fraction that contained core material substituted with several units of O-antigen building blocks. The chemical basis for these differences in reactivity remains to be established. As far as we know, this is the first report containing comprehensive immunochemical data on the LPS core of K. pneumoniae.

机译：测试了针对明尼苏达沙门氏菌R595的单克隆抗体（MAb），该单克隆抗体对内核的α-3-脱氧-D-甘露聚糖-辛酸（alpha-Kdo）具有特异性，与肺炎克雷伯菌的脂多糖（LPS）结合。在几种测定系统（酶联免疫吸附测定，被动溶血和抑制被动溶血）中对MAb进行了测试，其中包括代表所有九种目前已知的O型血清的肺炎克雷伯氏菌LPS（n = 23）。 MAb 20显示与肺炎克雷伯氏菌LPS的几乎所有O血清型都有反应性，并且该反应性可被合成Kdo抑制。这表明这些克雷伯氏菌LPS的核心中的表位非常类似于明尼苏达州LPS的核心中的表位。观察到属于同一O血清型的不同菌株的LPS之间的反应性差异很大。在LPS的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳后进行免疫印迹后，仅观察到MAb 20的反应性，其中快速移动的级分可能代表未取代的核心。对于包含被几个单元的O-抗原构建基团取代的核心材料的高分子量级分，未见结合。这些反应性差异的化学基础仍有待建立。据我们所知，这是第一份包含有关肺炎克雷伯菌的LPS核心的全面免疫化学数据的报告。

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《Infection and immunity》 |1994年第3期|共6页
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N M van der Meer; B J Appelmelk; A M Verweij-van Vught; W Nimmich; P Kosma; L G Thijs; J de Graaff; D M MacLaren;
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1. The tetrasaccharide L-alpha-D-heptose1-->2-L-alpha-D-heptose1--> 3-L-alpha-D-heptose1-->(3-deoxy-D-manno-octulosonic acid) and phosphate in lipid A define the conserved epitope in Haemophilus lipopolysaccharides recognized by a monoclonal antibody. [J] . S Borrelli, O Hegedus, D H Shaw, Infection and immunity . 1995,第9期

机译：四糖L-α-D-庚糖1->2-L-α-D-庚糖1->3-L-α-D-庚糖1->（3-脱氧-D-甘露糖辛酸）和磷酸盐脂质A中的“抗原”定义了被单克隆抗体识别的嗜血杆菌脂多糖中的保守表位。
2. The tetrasaccharide L-alpha-D-heptose1-->2-L-alpha-D-heptose1--> 3-L-alpha-D-heptose1-->(3-deoxy-D-manno-octulosonic acid) and phosphate in lipid A define the conserved epitope in Haemophilus lipopolysaccharides recognized by a monoclonal antibody. [J] . S Borrelli, O Hegedus, D H Shaw, Infection and immunity . 1995,第9期

机译：四糖L-α-D-庚糖1->2-L-α-D-庚糖1->3-L-α-D-庚糖1->（3-脱氧-D-甘露糖辛酸）和磷酸盐脂质A中的“抗原”定义了被单克隆抗体识别的嗜血杆菌脂多糖中的保守表位。
3. Endotoxin neutralization by an O-antigen specific monoclonal antibody: A potential novel therapeutic approach against Klebsiella pneumoniae ST258 [J] . Valéria Szijártó, Luis M. Guachalla, Katharina Hartl, Virulence. . 2017,第7期

机译：O抗原特异性单克隆抗体中和内毒素：针对肺炎克雷伯菌ST258的潜在新治疗方法
4. Epitope Mapping and Characterization of the Binding Specificity of Monoclonal Antibodies Directed Against Allergens of Grass Group 1 [C] . A. Petersen, G. Schramm, A. Kramer, Collegium Internationale Allergologicum . 2007

机译：针对草组过敏原的单克隆抗体结合特异性的表位测绘和表征
5. PRODUCTION AND EVALUATION OF ANTISERA AND MONOCLONAL ANTIBODIES DIRECTED AGAINST THE SEROVAR-SPECIFIC LIPOPOLYSACCHARIDE ANTIGENS OF VIBRIO CHOLERAE. [D] . ADAMS, LINDA BENTON. 1987

机译：针对霍乱弧菌的血清特异性脂多糖多糖抗原生产和评估的抗体和单克隆抗体。
6. Binding studies of a monoclonal antibody specific for 3-deoxy-D-manno-octulosonic acid with a panel of Klebsiella pneumoniae lipopolysaccharides representing all of the O serotypes. [O] . N M van der Meer, B J Appelmelk, A M Verweij-van Vught, 1994

机译：对3-脱氧-D-甘露糖辛酸具有特异性的单克隆抗体与一组代表所有O型血清型的肺炎克雷伯氏菌脂多糖的结合研究。
7. Binding to and Opsonophagocytic Activity of O-Antigen-Specific Monoclonal Antibodies against Encapsulated and Nonencapsulated Klebsiella pneumoniae Serotype O1 Strains [O] . Held, Thomas K., Jendrike, Nina R. M., Rukavina, Tomislav, 2000

机译：O抗原特异性抗封装和非封装肺炎克雷伯菌肺炎血清型O1菌株的结合和调理吞噬活性。
8. Phosphate Binding Specificities of Monoclonal Antibodies against Phosphoinositides in Liposomes [R] . Wassef, N. M., Roerdink, F., Swartz, G. M., 1984

机译：脂质体中抗磷脂酰肌醇单克隆抗体的磷酸结合特异性

Binding studies of a monoclonal antibody specific for 3-deoxy-D-manno-octulosonic acid with a panel of Klebsiella pneumoniae lipopolysaccharides representing all of the O serotypes.

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