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首页> 外文期刊>Infection and immunity >Enterotoxic activity of Klebsiella oxytoca cytotoxin in rabbit intestinal loops.
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Enterotoxic activity of Klebsiella oxytoca cytotoxin in rabbit intestinal loops.

机译：兔肠环中产酸克雷伯菌的细胞毒素的肠毒性。

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We examined the enterotoxicity of a Klebsiella oxytoca cytotoxin which is produced by K. oxytoca OK-1, a strain from a patient with antibiotic-associated hemorrhagic colitis. Injection of the cytotoxin into ligated ileal and colonic loops in rabbits caused the accumulation of fluid in the loops. The fluid was bloody in the ileal loops but not in the colonic ones. Histological examination revealed intense mucosal hemorrhage with erosion in the ileum, whereas no microscopic change was noted in the colon. The fluid accumulation was shown to be a dose-dependent response in both ileal and colonic loops. The amounts of the cytotoxin required for maximal fluid accumulation in ileal and colonic loops were 60 and 10 micrograms, respectively. Fluid accumulation was first noticeable in ileal loops 12 h and in colonic ones 5 h after the injection of these doses of the cytotoxin and then proceeded with time. When K. oxytoca OK-1, a cytotoxin-producing strain, was inoculated into the loops at doses of 1 x 10(8) and 5 x 10(9) CFU, similar fluid accumulation was observed. However, inoculation of K. oxytoca ATCC 13182, a non-cytotoxin-producing strain, at the same doses did not cause any change. These results suggest that the cytotoxin-producing strain of K. oxytoca is the causative organism of antibiotic-associated hemorrhagic colitis and that the toxin is the factor responsible for pathogenesis.
机译：我们检查了产氧克雷伯氏菌OK-1产生的产酸克雷伯菌细胞毒素的肠毒性，这是一种与抗生素相关的出血性结肠炎患者的菌株。将细胞毒素注射到结扎的回肠和结肠回路中的兔子中会导致回路中积聚液体。回肠loop中的液体是血腥的，但结肠回肠中没有。组织学检查显示回肠粘膜严重出血并伴有糜烂，而结肠未见镜下改变。在回肠和结肠循环中，液体积聚均显示为剂量依赖性反应。在回肠和结肠环中最大积聚液体所需的细胞毒素量分别为60和10微克。在注射这些剂量的细胞毒素后12 h，回肠loop中首先出现明显的积液，而在结肠中则在5 h出现积液，然后随着时间的流逝。当产毒素的产氧链球菌O​​K-1以1 x 10（8）和5 x 10（9）CFU的剂量接种到环中时，观察到相似的积液。但是，以相同剂量接种产非细胞毒素的产氧链球菌ATCC 13182不会引起任何变化。这些结果表明产氧克雷伯氏菌的细胞毒素菌株是抗生素相关性出血性结肠炎的病原体，并且毒素是引起发病的因素。

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