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首页> 外文期刊>Infection and immunity >Extractability of Cell Wall Polysaccharide from Lactobacilli and Streptococci by Autoclaving and by Dilute Acid
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Extractability of Cell Wall Polysaccharide from Lactobacilli and Streptococci by Autoclaving and by Dilute Acid

机译：高压灭菌法和稀酸法提取乳酸杆菌和链球菌细胞壁多糖的能力

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Autoclaving cell wall of Streptococcus mutans Ingbritt for 15 min under the Rantz and Randall conditions released one-tenth of the total cell wall carbohydrate, whereas two-thirds was extracted after autoclaving for 180 min. The extract contained the serotype c-specific antigen but lacked the lipoteichoic acid component extracted when whole cells were autoclaved. Autoclaving cell wall preparations from other strains of S. mutans and also Streptococcus salivarius and Streptococcus mitis in 0.85% NaCl for 180 min released the major proportion of the wall polysaccharide fraction. Approximately 50 to 90% of wall carbohydrate of Lactobacillus fermentum and Lactobacillus casei was released when cell wall preparations were autoclaved in 0.85% NaCl for 180 min. For wall preparations from several strains of S. mutans, autoclaving for 60 min at pH 3.75 released only 39 to 62% of wall carbohydrate, whereas almost total release could be achieved with the lactobacilli. Heating S. mutans Ingbritt cell wall for 24 h at 60°C in 0.1 N H2SO4 released only two-thirds of the wall carbohydrate; by comparison nearly all of the wall carbohydrate was released in 3 h from L. casei and L. fermentum. Autoclaving L. casei cell wall and purified soluble wall fractions hydrolyzed the phosphodiester bond between the polysaccharide and peptidoglycan. This was shown by the release of reactive N-acetylhexosamine in both cases and the presence of a phosphomonoester in the autoclaved soluble wall fractions. The results indicate that autoclaving can hydrolyze covalent linkages, and this must be considered when the Rantz and Randall procedure is used to obtain antigen preparations.
机译：在兰兹和兰德尔条件下，变形链球菌Ingbritt的高压灭菌细胞壁15分钟释放了总细胞壁碳水化合物的十分之一，而高压灭菌180分钟后提取了三分之二的碳水化合物。提取物含有血清型 c 特异性抗原，但缺少将全细胞高压灭菌后提取的脂磷壁酸成分。其他 S菌株的高压灭菌细胞壁制剂。变形链球菌以及唾液链球菌和链球菌在0.85％NaCl中浸泡180分钟，释放出壁多糖部分的主要部分。当将细胞壁制剂在0.85％NaCl中高压灭菌180分钟后，大约释放了发酵乳杆菌和干酪乳杆菌壁糖的50％至90％。用于从几种 S菌株制备墙体。在3.75 pH值下高压灭菌60分钟，仅释放39-62％的壁碳水化合物，而乳杆菌几乎可以完全释放。加热 S。在60°C下于0.1 N H 2 SO 4 中变形的Ingbritt细胞壁释放24小时，仅释放了壁中碳水化合物的三分之二。相比之下，几乎所有壁碳水化合物都在3小时内从 L中释放出来。 casei 和 L。发酵罐。高压灭菌。酪蛋白细胞壁和纯化的可溶性壁部分水解了多糖和肽聚糖之间的磷酸二酯键。这在两种情况下都通过反应性 N -乙酰己糖胺的释放和高压灭菌的可溶性壁馏分中存在的磷酸单酯来表明。结果表明，高压灭菌可以水解共价键，当使用Rantz和Randall程序获得抗原制剂时必须考虑这一点。

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