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首页> 外文期刊>Infection and immunity >Respective contributions to protection of primary and booster immunization with Escherichia coli heat-labile enterotoxin in rats.
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Respective contributions to protection of primary and booster immunization with Escherichia coli heat-labile enterotoxin in rats.

机译：分别对保护大鼠热不稳定肠毒素的初次和加强免疫的贡献。

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The respective contributions to protection of the route and dosage of primary and booster immunizations with Escherichia coli heat-labile enterotoxin were evaluated in rats. The degree of protection was determined by challenge with toxin and viable bacteria in ligated ileal loops, and the serum antitoxin response was assayed by enzyme-linked immunosorbent assay. Primary immunization was effective only when given by the parenteral route. The degree of protection was enhanced a fivefold dosage increase in the primary parenteral immunization in rats given constant dosages of booster immunizations either parenterally or perorally, but not by further dosage increases. In contrast, the degree of protection rose when dosages of the booster immunizations were increased over a 25-fold range. Four weekly peroral, but only two biweekly parenteral, booster immunizations were necessary to achieve strong protection; biweekly combined parenteral and peroral booster immunizations yielded both strong, immediate and extended protection. The degree of protection against the toxin correlated with that against viable bacteria and with elevated serum antitoxin titers: all seven groups with a protection index of greater than 5 against the toxin had strong protection against heat-labile toxin-producing strains and fourfold or greater increases in the antitoxin titers, whereas none of the nine groups with a protection index of less than 3 was protected against bacteria or had an equivalent antitoxin response. These observations show that once an adequate parenteral primary immunization is given, the degree of protection is influenced primarily by the dosage of the booster immunizations, the necessary number of which is dependent on their route of administration.
机译：在大鼠中评估了对大肠杆菌热不稳定肠毒素的初次和加强免疫途径和剂量保护的各自贡献。通过在结扎的回肠环中用毒素和活菌攻击来确定保护程度，并通过酶联免疫吸附测定法测定血清抗毒素反应。初次免疫仅在通过肠胃外途径进行时才有效。给定胃肠外或经口恒定剂量的加强免疫剂量，在大鼠初次肠胃外免疫中，保护程度提高了五倍，但没有进一步增加剂量。相反，当加强免疫的剂量增加25倍时，保护程度会提高。每周进行四次口服，但每两周一次两次肠胃外加强免疫是获得强有力的保护所必需的。每两周联合进行一次肠胃外和口服加强免疫，既能产生强大，即时和扩展的保护。对毒素的防护程度与对活细菌的防护程度相关，并且与血清抗毒素滴度升高相关：所有七个对毒素的防护指数均大于5的组均对产生热不稳定毒素的菌株具有较强的防护作用，并且增加四倍或更多。在抗毒素效价方面，其九个组的保护指数均小于3，而对细菌的保护或具有同等的抗毒素反应。这些观察结果表明，一旦给予足够的肠胃外初次免疫，保护程度主要受加强免疫的剂量影响，加强免疫的必要次数取决于其给药途径。

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