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首页> 外文期刊>Infection and immunity >Role of monocytes in polyclonal immunoglobulin production stimulated by sonicates of periodontally associated bacteria.
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Role of monocytes in polyclonal immunoglobulin production stimulated by sonicates of periodontally associated bacteria.

机译：单核细胞在牙周相关细菌的声波刺激下在多克隆免疫球蛋白生产中的作用。

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摘要

These studies were initiated to investigate monocyte regulation of polyclonal antibody responses of human peripheral blood lymphocytes stimulated by sonicates of periodontally associated bacteria. With pokeweed mitogen (PWM) as a positive reference, the role of monocytes in the peripheral blood lymphocyte response to Streptococcus sanguis and Wolinella HVS was examined by manipulating the number of monocytes and lymphocytes in culture. In comparison to PWM, optimal responses to the bacterial sonicates required very few monocytes (0.3% of the total cultured cells). Restoration of monocytes to physiological levels resulted in suppression of the response. PWM-stimulated responses were optimal at 5 to 15% monocyte content and were abolished after monocyte depletion. Individuals who were low responders or nonresponders to bacterial sonicates responded at normal levels after manipulation of monocyte concentration. Nonresponders produced normal levels of antibody when the monocyte concentration was reduced to 0.3% but were inhibited after monocyte reconstitution. The effects of monocyte concentration were tested over a wide dose range of bacterial sonicate and found to conform to the observed pattern throughout the dose range tested (10 to 1,000 micrograms/ml). The contrasting monocyte requirement of peripheral blood lymphocytes stimulated with PWM versus bacterial sonicates may reflect a quantitative difference in optimal macrophage concentration or may be due to a qualitative difference in lymphocyte-monocyte interactions in response to these activators.
机译：开展这些研究以研究由牙周相关细菌的声波刺激刺激的人外周血淋巴细胞多克隆抗体反应的单核细胞调节。以商陆有丝分裂原（PWM）为阳性参照，通过处理培养物中单核细胞和淋巴细胞的数量，检查了单核细胞在外周血淋巴细胞对血链球菌和沃林氏菌HVS的反应中的作用。与PWM相比，对细菌超声的最佳响应只需要很少的单核细胞（占培养细胞总数的0.3％）。单核细胞恢复到生理水平导致反应的抑制。 PWM刺激的响应在单核细胞含量为5％到15％时最佳，并且在单核细胞耗尽后被取消。对单音细胞浓度低反应者或对细菌超声反应无反应的个体在正常水平下反应。当单核细胞浓度降低至0.3％时，无反应者产生正常水平的抗体，但在单核细胞重建后被抑制。在很宽的细菌声波剂量范围内测试了单核细胞浓度的影响，发现在整个测试剂量范围内（10至1,000微克/毫升）都符合观察到的模式。 PWM刺激的外周血淋巴细胞与细菌声波刺激后的单核细胞需求相反，可能反映了最佳巨噬细胞浓度的定量差异，或者可能是由于对这些激活剂的淋巴细胞-单核细胞相互作用的定性差异。

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