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首页> 外文期刊>Infection and immunity >Chemical and immunochemical analyses of Bacteroides fragilis lipopolysaccharides.
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Chemical and immunochemical analyses of Bacteroides fragilis lipopolysaccharides.

机译：脆弱拟杆菌（Bacteroides fragilis）脂多糖的化学和免疫化学分析。

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Lipopolysaccharides (LPSs) from 17 different Bacteroides fragilis strains were extracted in a two-step procedure. The first step was a hot phenol-water extraction of whole bacteria, resulting in a crude aqueous phase, which after lyophilization in a second step was extracted with a phenol-chloroform-light petroleum mixture. The resulting LPSs, which were essentially free from contaminating nucleic acid, proteins, and capsular polysaccharide, were investigated for their qualitative and quantitative sugar and fatty acid composition, immunochemical specificity by enzyme-linked immunosorbent inhibition assays, and particle weight by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Of the 17 strains, 13 had LPSs which all contained L-rhamnose, D-glucose, D-galactose, and D-glucosamine in approximately the same ratio. Three of these LPSs also contained D-galactosamine. The fatty acid composition was also similar in that the same fatty acids, although in slightly varying proportions, were found in all LPSs. The 13 strains also showed the same specificity in inhibition studies by enzyme immunoassay with rabbit anti-LPS antisera and LPS antigen. The LPS particle weights were also very similar, in the range of what is found for LPSs from rough mutant strains of enterobacteria. Our results suggest that most strains of B. fragilis have similar, if not identical, LPSs with relatively short polysaccharide chains.
机译：通过两步过程从17种不同的脆弱拟杆菌中提取脂多糖（LPS）。第一步是对全部细菌进行热酚水萃取，得到粗水相，第二步冻干后，将其用苯酚-氯仿-轻质石油混合物萃取。对所得的LPS基本上不含污染的核酸，蛋白质和荚膜多糖进行了定性和定量的糖和脂肪酸组成，酶联免疫吸附抑制试验的免疫化学特异性以及十二烷基硫酸钠的颗粒重量研究-聚丙烯酰胺凝胶电泳。在17个菌株中，有13个具有LPS，它们全部以大致相同的比例包含L-鼠李糖，D-葡萄糖，D-半乳糖和D-葡萄糖胺。这些LPS中的三个还含有D-半乳糖胺。脂肪酸组成也相似，尽管在所有LPS中发现相同的脂肪酸，尽管比例略有不同。通过用兔抗LPS抗血清和LPS抗原进行酶免疫测定，这13株菌株在抑制研究中也显示出相同的特异性。 LPS颗粒的重量也非常相似，在肠道细菌的粗糙突变菌株中发现的LPS的范围内。我们的结果表明，大多数脆弱型芽孢杆菌菌株具有相似的（即使不是相同的）具有相对较短的多糖链的LPS。

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