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首页> 外文期刊>Infection and immunity >Elaboration of a 3.6-kilodalton lipooligosaccharide, antibody against which is absent from human sera, is associated with serum resistance of Neisseria gonorrhoeae.
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Elaboration of a 3.6-kilodalton lipooligosaccharide, antibody against which is absent from human sera, is associated with serum resistance of Neisseria gonorrhoeae.

机译：制备3.6千达尔顿的脂寡糖（人血清中不存在针对该抗体的抗体）与淋病奈瑟氏球菌的血清耐药性有关。

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Neisseria gonorrhoeae strains that resist lysis by normal human sera (NHS) do so, in part, because NHS contain immunoglobulin M (IgM) specific for lipooligosaccharide (LOS) antigens of serum-sensitive strains, but lack antibodies for LOS antigens that can serve as loci for immune lysis of serum-resistant (serr) strains. We used a monoclonal antibody (McAb), specific for an epitope within a 3.6-kilodalton (kDa) component of Neisseria meningitidis L8 LOS, that binds a 3.6-kDa gonococcal LOS component so that we could explore further serr gonococcal strains. The McAb bound to the LOS of 6 of 7 serr of strains but not to the LOS of 0 of 14 serum-sensitive and serum-intermediate gonococcal strains of diverse origin. We studied three serr strains further. Strain 7134 does not elaborate the 3.6-kDa LOS component and does not bind the McAb; strains WR220 and WR302 do elaborate the 3.6-kDa LOS component. The titer (log2) at which the McAb, diluted in NHS, lysed strain WR220 was 7.7; for WR302 it was 3.7, and for 7134 it was 0. Addition of McAb to NHS caused increased classical and alternative-pathway C3 deposition onto strain WR220, but only classical-pathway-activated C3 deposition onto strain WR302. The difference in lytic effectiveness of the McAb for the two strains, therefore, may result from differences in alternative-pathway augmentation of McAb-dependent classical-pathway activation on their surfaces. None of 40 randomly selected normal young adults had serum antibody that could compete with the McAb for binding to WR220 LOS in a solid-phase RIA. We conclude that the 3.6-kDa LOS component is commonly expressed by serr strains of N. gonorrhoeae and that antibody to it would be lytic if present in human serum, but that it is infrequently, if ever, present. As a result, strains elaborating this LOS are resistant to lysis by NHS.
机译：耐正常人血清（NHS）溶解的淋病奈瑟氏球菌之所以会这样做，部分是因为NHS包含对血清敏感菌株的脂寡糖（LOS）抗原具有特异性的免疫球蛋白M（IgM），但缺少可作为LOS抗原的抗体用于血清抗性（serr）菌株免疫裂解的基因座。我们使用了单克隆抗体（McAb），该抗体对脑膜炎奈瑟氏球菌L8 LOS的3.6千达尔顿（kDa）组件内的表位具有特异性，该抗体结合3.6 kDa的淋球菌LOS组件，以便我们可以探索进一步的Serr淋球菌菌株。 McAb结合7种Serr菌株中的6种的LOS，但不结合14种不同来源的血清敏感性和血清中间性淋球菌菌株中的0种的LOS。我们进一步研究了三种Serr菌株。菌株7134没有详述3.6-kDa的LOS组分，并且不结合McAb。菌株WR220和WR302确实完成了3.6 kDa的LOS成分。在NHS中稀释的McAb裂解的菌株WR220的效价（log2）为7.7;对于WR302，它是3.7，对于7134，它是0。向NHS中添加McAb导致在菌株WR220上经典和替代途径C3沉积增加，但在菌株WR302上仅经典途径激活的C3沉积。因此，对于两种菌株，McAb的裂解效力不同，可能是由于其表面上依赖于McAb的经典途径激活的替代途径增加的差异所致。在随机选择的40名正常年轻人中，没有一个能够与McAb竞争在固相RIA中与WR220 LOS结合的血清抗体。我们得出的结论是，3.6 kDa LOS成分通常由淋病奈瑟氏球菌的serr菌株表达，并且如果存在于人血清中，它的抗体会溶解，但是很少存在（如果有的话）。结果，精心制作此LOS的菌株可抵抗NHS的裂解。

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