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首页> 外文期刊>Infection and immunity >Antibody-independent and -dependent opsonization of group B Streptococcus requires the first component of complement C1.
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Antibody-independent and -dependent opsonization of group B Streptococcus requires the first component of complement C1.

机译：B组链球菌的抗体非依赖性和调理作用需要补体C1的第一部分。

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The role of the classical complement pathway and specifically the first component, C1 in antibody-independent opsonization of type Ia group B Streptococcus (GBS) was investigated. For these studies a radiolabeled bacterial uptake assay was developed that was dependent on time and bacterial concentration and that required an intact classical complement pathway. To directly investigate the role of C1 in opsonization of type Ia GBS, C1 was isolated by chromatography on an immunoglobulin G (IgG) affinity column and further purified by molecular sieve chromatography on an Ultrogel AcA 22 column. When normal human serum was absorbed with 10(9) CFU of type Ia or III GBS, the serum opsonic capacity diminished (33 to 34%) for type Ia GBS compared with unadsorbed serum. Preincubation of the bacteria with purified C1 (10(4)U of C1 per ml) restored the opsonizing capacity of the adsorbed serum. A C1-depleted serum was prepared from the nonadherent fractions of the CH-sepharose 4B IgG column which only contained 5 U of C1 per ml. Substitution of C1-depleted reagent for normal serum in the uptake assay resulted in dramatic decreases in the opsonization of type Ia GBS, but opsonization could be restored by preincubation of the bacteria with purified C1. Heat-inactivated C1 depleted serum did not support opsonization of type Ia GBS, even with the addition of C1. Preincubation of type Ia GBS with heat-inactivated hyperimmune sera did not result in opsonization of type Ia GBS in the presence of C1-depleted serum. However, opsonization could be restored by the addition of C1, and the effects of C1 and antibody were additive. These results indicate the critical role of C1 in direct activation of the classical complement pathway by type Ia GBS and in antibody-mediated opsonization of the bacteria.
机译：研究了经典补体途径，特别是第一个成分C1在Ia型B群链球菌（GBS）的抗体非依赖性调理作用中的作用。对于这些研究，开发了放射性标记的细菌吸收测定法，该测定法取决于时间和细菌浓度，并且需要完整的经典补体途径。为了直接研究C1在Ia型GBS调理作用中的作用，在免疫球蛋白G（IgG）亲和柱上通过色谱分离C1，并在Ultrogel AcA 22柱上通过分子筛色谱进一步纯化。当正常人血清被Ia或III型GBS的10（9）CFU吸收时，与未吸收的血清相比，Ia GBS的血清调理能力降低了（33％至34％）。将细菌与纯化的C1（每毫升10（4）U C1）进行预培养，可恢复吸附血清的调理能力。由CH-琼脂糖4B IgG柱的非粘附级分制备了C1贫血的血清，每毫升仅含5 U C1。在摄取分析中用C1耗竭的试剂代替正常血清会导致Ia型GBS的调理作用显着降低，但是可以通过将细菌与纯化的C1预孵育来恢复调理作用。即使添加了C1，热灭活的C1贫血血清也不支持Ia型GBS的调理作用。在缺少C1的血清存在下，将Ia型GBS与热灭活的超免疫血清一起预孵育不会导致Ia型GBS的调理作用。但是，加入C1可以恢复调理作用，并且C1和抗体的作用是累加的。这些结果表明，C1在Ia型GBS直接激活经典补体途径和细菌介导的调理作用中起着关键作用。

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