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首页> 外文期刊>Infection and immunity >A Mycobacterium leprae gene encoding a fibronectin binding protein is used for efficient invasion of epithelial cells and Schwann cells.
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A Mycobacterium leprae gene encoding a fibronectin binding protein is used for efficient invasion of epithelial cells and Schwann cells.

机译：编码纤连蛋白结合蛋白的麻风分枝杆菌基因用于有效侵袭上皮细胞和雪旺氏细胞。

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Mycobacterium leprae, the causative agent of leprosy, is an obligate intracellular pathogen. M. leprae can infect a variety of cells in vivo, including epithelial cells, muscle cells, and Schwann cells, in addition to macrophages. The ligand-receptor interactions important in the attachment and ingestion of M. leprae by these nonmacrophage cells remains unknown. Fibronectin (FN) significantly enhances both attachment and ingestion of M. leprae by epithelial and Schwann cell lines. We cloned an M. leprae FN binding protein (FN attachment protein [FAP]) distinct from the 85ABC complex which has been shown previously to bind FN. The FAP open reading frame predicts a protein of 29.5 kDa with a 39-amino-acid signal peptide and was previously described as an antigen in leprosy patients. M. leprae FAP has homologies in M. vaccae, M. avium, and M. tuberculosis, as determined by Southern blotting and direct peptide analysis. Both anti-FAP antibodies and an Escherichia coli-expressed recombinant protein significantly blocked M. leprae attachment and internalization by T-24, an epithelial cell line, and JS1, a Schwann cell line. These data suggest that FN can be a bridging opsonic ligand for attachment of mycobacteria to nonphagocytes and that FAP plays an important role in this process. This may be an important step in the initiation of M. leprae infection in vivo.
机译：麻风分枝杆菌是麻风的病原体，是专性的细胞内病原体。麻风分枝杆菌除巨噬细胞外还可以感染多种体内细胞，包括上皮细胞，肌肉细胞和雪旺氏细胞。这些非巨噬细胞附着和摄取麻风分枝杆菌重要的配体-受体相互作用仍然未知。纤连蛋白（FN）显着增强了麻风支原体通过上皮和施万细胞系的附着和摄取。我们克隆了一个与85ABC复合体不同的麻风分枝杆菌FN结合蛋白（FN附着蛋白[FAP]），后者先前已显示出可以结合FN。 FAP开放阅读框预测具有39个氨基酸信号肽的29.5 kDa蛋白，先前被描述为麻风患者的抗原。通过Southern印迹法和直接肽分析法测定，麻风分枝杆菌FAP在牛分枝杆菌，鸟分枝杆菌和结核分枝杆菌中具有同源性。抗FAP抗体和表达大肠杆菌的重组蛋白都通过T-24（上皮细胞系）和JS1（Schwann细胞系）显着阻断了麻风分枝杆菌的附着和内在化。这些数据表明，FN可以是桥接分枝配体，使分枝杆菌附着于非吞噬细胞，FAP在此过程中起着重要作用。这可能是体内启动麻风分枝杆菌感染的重要步骤。

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