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首页> 外文期刊>Infection and immunity >Generation of interleukin-8 from human monocytes in response to Trichomonas vaginalis stimulation.
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Generation of interleukin-8 from human monocytes in response to Trichomonas vaginalis stimulation.

机译：从人单核细胞响应阴道毛滴虫的刺激产生白介素8。

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Neutrophils are the predominant inflammatory cells found in the vaginal discharges of patients with Trichomonas vaginalis infection. We have investigated the possible role of interleukin-8 (IL-8) in the inflammatory response elicited by T. vaginalis infection. This study has shown that T. vaginalis induces blood monocytes to produce large amounts of bioactive IL-8, mainly by membrane components of T. vaginalis (MTV). Monocyte-derived IL-8 induced by MTV was dose and time dependent. The peak level of IL-8 was 102 +/- 11 ng/ml of conditioned media (mean +/- standard error; n = 5) obtained from MTV-stimulated monocytes (MTVCM) at 36 h of cultivation. With a multichamber chemotactic assay, we found an optimal neutrophil chemotaxis (177 +/- 14 migrated cells) induced by MTVCM collected at 16 h of cultivation when the level of IL-8 was 42 +/- 8 ng/ml. A neutralizing monoclonal antibody directed against IL-8, but not the irrelevant antibodies, significantly blocked the neutrophil chemotactic activity (decreased from 153 +/- 6 to 23 +/- 3 migrated cells; n = 3 [P < 0.001]) induced by MTVCM. Moreover, the maximum increase of the IL-8 mRNA level from MTV-treated monocytes was observed after a 5-h cultivation and decreased thereafter. Monocytes cocultured with MTV in the presence of a neutralizing monoclonal antibody directed against tumor necrosis factor alpha, but not against IL-1 beta, decreased IL-8 production by 25% (P < 0.05), indicating that the release of IL-8 in MTV-stimulated monocytes is partially dependent on tumor necrosis factor alpha. The capacity of MTV-induced monocytes to synthesize IL-8 suggests that these cells can contribute to the induction of the acute inflammatory response seen in T. vaginalis infection.
机译：中性粒细胞是在阴道毛滴虫感染患者的阴道分泌物中发现的主要炎症细胞。我们已经研究了白细胞介素8（IL-8）在阴道锥虫感染引起的炎症反应中的可能作用。这项研究表明，阴道锥虫主要通过阴道锥虫（MTV）的膜成分诱导血液单核细胞产生大量生物活性IL-8。 MTV诱导的单核细胞衍生的IL-8具有剂量和时间依赖性。在培养36小时后，从MTV刺激的单核细胞（MTVCM）获得的IL-8的峰值水平为102 +/- 11 ng / ml的条件培养基（平均+/-标准误差; n = 5）。通过多室趋化分析，我们发现在培养16 h时IL-8水平为42 +/- 8 ng / ml时，由MTVCM诱导的最佳嗜中性白细胞趋化性（177 +/- 14个迁移细胞）。针对IL-8的中和性单克隆抗体，而不是不相关的抗体，显着阻断了中​​性粒细胞的趋化活性（从153 +/- 6降低至23 +/- 3迁移的细胞; n = 3 [P <0.001]）。 MTVCM。此外，在培养5小时后，观察到了经MTV处理的单核细胞IL-8 mRNA水平的最大增加，此后减少了。在针对肿瘤坏死因子α而非IL-1 beta的中和性单克隆抗体的存在下，与MTV共培养的单核细胞使IL-8的产生降低了25％（P <0.05），表明IL-8的释放MTV刺激的单核细胞部分依赖于肿瘤坏死因子α。 MTV诱导的单核细胞合成IL-8的能力表明，这些细胞可有助于诱导阴道锥虫感染中出现的急性炎症反应。

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