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首页> 外文期刊>Infection and immunity >Elevated aspartic proteinase secretion and experimental pathogenicity of Candida albicans isolates from oral cavities of subjects infected with human immunodeficiency virus.
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Elevated aspartic proteinase secretion and experimental pathogenicity of Candida albicans isolates from oral cavities of subjects infected with human immunodeficiency virus.

机译：从感染人免疫缺陷病毒的受试者的口腔中分离出白色念珠菌的天冬氨酸蛋白酶分泌和实验致病性。

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摘要

Isolates of Candida albicans from the oral cavities of subjects at different stages of human immunodeficiency virus (HIV) infection or uninfected controls were examined for (i) production of aspartic proteinase(s), a putative virulence-associated factor(s); (ii) the presence in the fungal genome of two major genes (SAP1 and SAP2) of the aspartic proteinase family; and (iii) experimental pathogenicity in a murine model of systemic infection. It was found that the fungal isolates from symptomatic patients secreted, on average, up to eightfold more proteinase than the isolates from uninfected or HIV-infected but asymptomatic subjects. This differential property was stably expressed by the strains even after years of maintenance in stock cultures. Moreover, representative high-proteinase isolates were significantly more pathogenic for mice than low-proteinase isolates of C. albicans. The characters high proteinase and increased virulence were not associated with a single molecular type or category identifiable through DNA fingerprinting or pulsed-field electrophoretic karyotype, and both SAP1 and SAP2 genes were present in both categories of isolates, on the same respective chromosomes. In conclusion, our data suggest that during HIV infection more-virulent strains or biotypes of C. albicans which are identifiable by direct analysis of virulence determinants are selected. It also appears that the biotype switch to increased aspartic proteinase and virulence properties occurs before the HIV-infected subject enters the symptomatic stage and overt AIDS.
机译：从人类免疫缺陷病毒（HIV）感染或未感染对照的不同阶段的受试者口腔中分离出白色念珠菌，检查（i）天冬氨酸蛋白酶的产生，这是一种假定的毒力相关因子。 （ii）在真菌基因组中存在天冬氨酸蛋白酶家族的两个主要基因（SAP1和SAP2）; （iii）在小鼠全身感染模型中的实验致病性。已发现，有症状患者的真菌分离物平均分泌的蛋白酶比未感染或HIV感染但无症状受试者的分离菌多出八倍。即使在原种培养中保持多年后，这种差异性也能通过菌株稳定表达。此外，代表性的高蛋白酶分离株对小鼠的致病性比白色念珠菌的低蛋白酶分离株高。高蛋白酶和高毒力的特征与通过DNA指纹图谱或脉冲场电泳核型可鉴定的单个分子类型或类别无关，SAP1和SAP2基因均存在于同一菌株各自的染色体上。总之，我们的数据表明，在HIV感染过程中，选择了可通过直接测定毒力决定因素确定的更毒力的白色念珠菌菌株或生物型。还似乎是，在HIV感染的受试者进入症状期和明显的AIDS之前，生物型转向增加的天冬氨酸蛋白酶和毒力特性已经发生。

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