Oral immunization with an attenuated vaccine strain of Salmonella typhimurium expressing the serine-rich Entamoeba histolytica protein induces an antiamebic immune response and protects gerbils from amebic liver abscess.

T Zhang; S L Stanley

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Oral immunization with an attenuated vaccine strain of Salmonella typhimurium expressing the serine-rich Entamoeba histolytica protein induces an antiamebic immune response and protects gerbils from amebic liver abscess.

机译：表达减毒疫苗的鼠伤寒沙门氏菌减毒株表达富含丝氨酸的Entomoeba histolytica蛋白进行口服免疫，可诱导抗阿米巴免疫反应并保护沙鼠免受阿米巴肝脓肿的侵袭。

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Attenuated salmonellae represent attractive candidates for the delivery of foreign antigens by oral vaccination. In this report, we describe the high-level expression of a recombinant fusion protein containing the serine-rich Entamoeba histolytica protein (SREHP), a protective antigen derived from virulent amebae, and a bacterially derived maltose-binding protein (MBP) in an attenuated strain of Salmonella typhimurium. Mice and gerbils immunized with S. typhimurium expressing SREHP-MBP produced mucosal immunoglobulin A antiamebic antibodies and serum immunoglobulin G antiamebic antibodies. Gerbils vaccinated with S typhimurium SREHP-MBP were protected against amebic liver abscess, the most common extraintestinal complication of amebiasis. Our findings indicate that the induction of mucosal and immune responses to the amebic SREHP antigen is dependent on the level of SREHP-MBP expression in S. typhimurium and establish that oral vaccination with SREHP can produce protective immunity to invasive amebiasis.

机译：减毒沙门氏菌代表了通过口服疫苗递送外来抗原的有吸引力的候选物。在本报告中，我们描述了重组融合蛋白的高水平表达，该融合蛋白在减毒中包含富含丝氨酸的组织变形虫Entamoeba组织溶蛋白（SREHP），有毒的变形虫衍生的保护性抗原和细菌衍生的麦芽糖结合蛋白（MBP）。鼠伤寒沙门氏菌菌株。用表达SREHP-MBP的鼠伤寒沙门氏菌免疫的小鼠和沙鼠产生了粘膜免疫球蛋白A抗贫抗体和血清免疫球蛋白G抗贫抗体。接种鼠伤寒沙门氏菌SREHP-MBP的沙鼠可以预防阿米巴肝脓肿，这是阿米巴病最常见的肠外并发症。我们的发现表明，对阿米巴SREHP抗原的粘膜和免疫反应的诱导取决于鼠伤寒沙门氏菌SREHP-MBP表达的水平，并证明口服SREHP疫苗可产生针对侵袭性阿米巴病的保护性免疫。

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《Infection and immunity》 |1996年第5期|共6页
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T Zhang; S L Stanley;
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中图分类医学免疫学;
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1. Oral immunization with an attenuated vaccine strain of Salmonella typhimurium expressing the serine-rich Entamoeba histolytica protein induces an antiamebic immune response and protects gerbils from amebic liver abscess. [J] . T Zhang, S L Stanley Infection and immunity . 1996,第5期

机译：表达减毒疫苗的鼠伤寒沙门氏菌减毒株表达富含丝氨酸的Entomoeba histolytica蛋白进行口服免疫，可诱导抗阿米巴免疫反应并保护沙鼠免受阿米巴肝脓肿的侵袭。
2. Oral immunization with attenuated vaccine strains of Vibrio cholerae expressing a dodecapeptide repeat of the serine-rich Entamoeba histolytica protein fused to the cholera toxin B subunit induces systemic and mucosal antiamebic and anti-V. cholerae antibody responses in mice. [J] . E T Ryan, J R Butterton, T Zhang, Infection and immunity . 1997,第8期

机译：表达与融合霍乱毒素B亚基的富含丝氨酸的Entamoeba histolytica蛋白的十二肽重复序列的霍乱弧菌减毒疫苗株进行口服免疫，可诱导全身性和粘膜性抗贫血和抗V。小鼠的霍乱抗体反应。
3. Oral immunization with attenuated vaccine strains of Vibrio cholerae expressing a dodecapeptide repeat of the serine-rich Entamoeba histolytica protein fused to the cholera toxin B subunit induces systemic and mucosal antiamebic and anti-V. cholerae antibody responses in mice. [J] . E T Ryan, J R Butterton, T Zhang, Infection and immunity . 1997,第8期

机译：表达与融合霍乱毒素B亚基的富含丝氨酸的Entamoeba histolytica蛋白的十二肽重复序列的霍乱弧菌减毒疫苗株进行口服免疫，可诱导全身性和粘膜性抗贫血和抗V。小鼠的霍乱抗体反应。
4. Oral immunization with an attenuated vaccine strain of Salmonella typhimurium expressing the serine-rich Entamoeba histolytica protein induces an antiamebic immune response and protects gerbils from amebic liver abscess. [O] . T Zhang, S L Stanley Jr 1996

机译：表达减毒疫苗的鼠伤寒沙门氏菌减毒株表达富含丝氨酸的变形杆菌Entamoeba组织溶蛋白可进行抗阿米巴免疫反应并保护沙鼠免受阿米巴肝脓肿的侵害。
5. Oral immunization with an attenuated vaccine strain of Salmonella typhimurium expressing the serine-rich Entamoeba histolytica protein induces an antiamebic immune response and protects gerbils from amebic liver abscess [O] . T Zhang, S L Stanley 1996

机译：口服免疫疫苗菌株的沙门氏菌毒蕈氏菌，表达富含丝氨酸的Entamoeba组织醇素蛋白诱导抗杀伤性免疫应答并保护来自琥珀肝脏脓肿的恶果

Oral immunization with an attenuated vaccine strain of Salmonella typhimurium expressing the serine-rich Entamoeba histolytica protein induces an antiamebic immune response and protects gerbils from amebic liver abscess.

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