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首页> 外文期刊>Infection and immunity >Identification of neutralization and diagnostic epitopes on PIM, the polymorphic immunodominant molecule of Theileria parva.
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Identification of neutralization and diagnostic epitopes on PIM, the polymorphic immunodominant molecule of Theileria parva.

机译：鉴定PIM（Theileria parva的多态免疫优势分子）上的中和表位和诊断表位。

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The polymorphic immunodominant molecule (PIM) of Theileria parva is expressed by the schizont and sporozoite stages of the parasite. We have recently cloned the cDNA encoding the PIM antigen from two stocks of the parasite: the cattle-derived T. parva (Muguga) stock and a buffalo-derived stock. The cDNAs were used in transient-transfection assays to assess the reactivity of the antigen with monoclonal antibodies (MAb) previously raised against schizont-infected cells and used for parasite strain identification. We demonstrate that 19 of the 25 MAb are specific for PIM. Antibody reactivities with deletion mutants of a fusion protein containing PIM and Pepscan analysis of the Muguga version of the molecule with 13 of the MAb indicate that there are at least 10 different epitopes throughout the molecule. None of the MAb react with a tetrapeptide repeat present in the central region of the molecule, probably because of an inability of BALB/c mice to produce antibodies to this repeat. In contrast, sera from infected cattle react strongly with the repeat region, suggesting that this region alone may be useful as a diagnostic reagent. Previous studies showed that MAb to PIM inhibit sporozoite infectivity of bovine lymphocytes in vitro, which suggests that the antigen may be useful in immunizing cattle against T. parva infection. Pepscan analysis revealed that sera from infected cattle reacted with peptides recognized by the neutralizing MAb, as did sera from cattle inoculated with a PIM-containing recombinant protein. The latter sera did not, however, neutralize sporozoite infectivity in vitro. These results will be useful in exploiting the strain identification, diagnostic, and immunizing potentials of this family of antigens.
机译：泰勒虫小体的多态免疫优势分子（PIM）由寄生虫的裂殖体和子孢子阶段表达。我们最近从两种寄生虫种群中克隆了编码PIM抗原的cDNA：来自牛的T. parva（Muguga）种群和一个来自水牛的种群。将这些cDNA用于瞬时转染测定中，以评估抗原与先前针对裂殖体感染细胞产生的单克隆抗体（MAb）的反应性，并用于鉴定寄生虫菌株。我们证明了25 MAb中的19种是针对PIM的。带有PIM融合蛋白的缺失突变体的抗体反应性以及对Muguga版本的分子和13个MAb的Pepscan分析表明，整个分子中至少有10个不同的表位。没有一个MAb与存在于分子中心区域的四肽重复序列发生反应，可能是因为BALB / c小鼠无法产生针对该重复序列的抗体。相反，来自被感染牛的血清与重复区域发生强烈反应，表明仅该区域可以用作诊断试剂。先前的研究表明，针对PIM的单克隆抗体在体外可抑制牛淋巴细胞的子孢子感染性，这表明该抗原可用于免疫牛抗T. parva感染。胃蛋白酶分析显示，被感染牛的血清与被中和单克隆抗体识别的肽反应，来自接种含PIM的重组蛋白的牛血清也是如此。但是，后者的血清不能在体外中和子孢子的感染性。这些结果将有助于开发该抗原家族的菌株鉴定，诊断和免疫潜能。

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