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首页> 外文期刊>Infection and immunity >Characterization, occurrence, and molecular cloning of a 39-kilodalton Brucella abortus cytoplasmic protein immunodominant in cattle.
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Characterization, occurrence, and molecular cloning of a 39-kilodalton Brucella abortus cytoplasmic protein immunodominant in cattle.

机译：牛中39公斤的流产布鲁氏菌流产细胞质蛋白的免疫性鉴定，发生和分子克隆。

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摘要

Monoclonal antibodies and polyclonal antisera recognizing a 39-kDa protein (P39) of brucellin, a cytoplasmic extract from Brucella melitensis rough strain B115, were produced. The P39 was purified by anion-exchange chromatography. Eleven of fourteen Brucella-infected cows whose infections had been detected by the delayed-type hypersensitivity (DTH) test with brucellergen also developed a DTH reaction when purified P39 was used as the trigger. The T-cell proliferative responses to P39 of peripheral blood lymphocytes from Brucella-infected cows were also positive. None of the animals infected with other bacterial species that are presumed to induce immunological cross-reactions with Brucella spp. reacted to P39, either in DTH tests or in lymphocyte proliferation assays. A lambda gt11 genomic library of Brucella abortus was screened with a monoclonal antibody specific for P39, and the gene coding for this protein was subsequently isolated. The nucleotide sequence of the P39 gene was determined, and the deduced amino acid sequence is in accordance with the sequence of an internal peptide isolated from P39.
机译：产生了识别布鲁氏菌素（一种来自布鲁氏菌粗糙菌株B115的细胞质提取物）的39 kDa蛋白（P39）的单克隆抗体和多克隆抗血清。通过阴离子交换色谱法纯化P39。当使用纯化的P39触发时，通过布鲁氏菌病的迟发型超敏反应（DTH）测试检测出感染的14头布鲁氏菌感染的母牛中，也发生了DTH反应。来自布鲁氏菌感染的母牛的外周血淋巴细胞对P39的T细胞增殖反应也为阳性。推测没有其他动物感染的动物被认为可诱导与布鲁氏菌的免疫交叉反应。在DTH试验或淋巴细胞增殖试验中对P39有反应。用对P39特异的单克隆抗体筛选流产布鲁氏菌的gt11基因组文库，随后分离出编码该蛋白的基因。确定了P39基因的核苷酸序列，推导的氨基酸序列与从P39分离的内部肽的序列一致。

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