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首页> 外文期刊>Infection and immunity >Characterization of an Acidic-pH-Inducible Stress Protein (hsp70), a Putative Sulfatide Binding Adhesin, fromHelicobacter pylori
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Characterization of an Acidic-pH-Inducible Stress Protein (hsp70), a Putative Sulfatide Binding Adhesin, fromHelicobacter pylori

机译：酸性pH诱导的应激蛋白（hsp70），一种幽门螺杆菌的硫化物结合粘附素的表征

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摘要

The in vitro glycolipid binding specificity of the gastric pathogenHelicobacter pylori is altered to include sulfated glycolipids (sulfatides) following brief exposure of the organism to acid pH typical of the stomach. This change is prevented by anti-hsp70 antibodies, suggesting that hsp70 may be a stress-induced surface adhesin, mediating sulfatide recognition. To facilitate investigation of the role of hsp70 in attachment, we have cloned and sequenced theH. pylori hsp70 gene (dnaK). Thehsp70 gene was identified by probing a cosmid DNA library made from H. pylori 439 with a PCR amplicon generated with oligonucleotides synthesized to highly conserved regions ofdnaK. The 1.9-kb H. pylori hsp70 gene encodes a product of 616 amino acids. Primer extension analysis revealed a single transcription start site, while Northern blot analysis established that hsp70 was preferentially induced by low pH rather than by heat shock. The ability ofH. pylori to alter its glycolipid binding specificity following exposure to low pH by upregulating hsp70 and by expressing hsp70 on the bacterial surface may provide a survival advantage during periods of high acid stress.
机译：胃病原体幽门螺杆菌的体外糖脂结合特异性在有机体短暂暴露于典型的胃酸pH值后已改变为包括硫酸化的糖脂（硫酸脂）。抗hsp70抗体可防止这种变化，表明hsp70可能是应激诱导的表面粘附素，介导了硫化物的识别。为了便于研究hsp70在附着中的作用，我们克隆并测序了 H。幽门螺杆菌hsp70 基因（ dnaK ）。通过探测由 H制成的粘粒DNA文库，鉴定了 hsp70 基因。 pylori 439，其PCR扩增子是由合成至 dnaK 高度保守区域的寡核苷酸产生的。 1.9 kb H。幽门螺杆菌hsp70 基因编码616个氨基酸。引物延伸分析揭示了一个转录起始位点，而Northern印迹分析确定 hsp70 优先由低pH而不是热激诱导。 H的能力。幽门螺杆菌通过上调 hsp70 并在细菌表面表达hsp70而改变其在低pH条件下的糖脂结合特异性，可在高酸胁迫期间提供生存优势。

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