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首页> 外文期刊>Infection and immunity >Immunoglobulins to Group A Streptococcal Surface Molecules Decrease Adherence to and Invasion of Human Pharyngeal Cells
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Immunoglobulins to Group A Streptococcal Surface Molecules Decrease Adherence to and Invasion of Human Pharyngeal Cells

机译：A组链球菌表面分子的免疫球蛋白会降低对人咽细胞的粘附和侵袭。

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The M protein is one of the most important virulence factors of group A streptococci (Streptococcus pyogenes) and may play an important role in the first steps of streptococcal infection. Since acute pharyngitis is a frequently occurring infectious disease caused by these bacteria, we wished to know whether antibodies to the M protein or other surface components inhibit adherence and internalization of streptococci to pharyngeal cells. We investigated the role of whole human secretory immunoglobulin A (sIgA), M6 protein-specific sIgA, and M6 protein-specific serum IgG in the inhibition of streptococcal adherence and internalization to cultured human pharyngeal cells. S. pyogenes D471, which produces a type 6 M protein (M+), and its isogenic M-negative (M?) derivative JRS75 were tested. Purified whole sIgA, M protein-specific sIgA, and sIgA preabsorbed with M protein were able to decrease significantly the adherence of streptococci to pharyngeal cells. Purified IgG against the M6 protein did not diminish the attachment of streptococci to the pharyngeal cells but did reduce internalization. Thus, our data suggest that secretory IgA may play a key role in preventing streptococcal infection at mucosal surfaces by blocking adherence while affinity-purified anti-M protein-specific IgG blocks epitopes responsible for invasion.
机译：M蛋白是A族链球菌（化脓性链球菌）的最重要毒力因子之一，可能在链球菌感染的第一步中起重要作用。由于急性咽炎是由这些细菌引起的常见传染病，因此我们希望知道针对M蛋白或其他表面成分的抗体是否抑制链球菌对咽细胞的粘附和内在化。我们调查了整个人类分泌型免疫球蛋白A（sIgA），M6蛋白特异性sIgA和M6蛋白特异性血清IgG在抑制链球菌粘附和内化培养人咽细胞中的作用。 S。测试了能产生6 M型蛋白（M +）的化脓性菌D471及其同基因M阴性（M2）衍生物JRS75。纯化的完整sIgA，M蛋白特异性sIgA和预先吸收有M蛋白的sIgA能够显着降低链球菌对咽细胞的粘附。针对M6蛋白的纯化IgG不会减少链球菌对咽细胞的附着，但会减少内化作用。因此，我们的数据表明，分泌型IgA可能通过阻止粘附而在黏膜表面预防链球菌感染中发挥关键作用，而亲和纯化的抗M蛋白特异性IgG则阻断了负责侵袭的表位。

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