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首页> 外文期刊>Infection and immunity >Selective Recruitment of T-Cell Subsets to the Udder during Staphylococcal and Streptococcal Mastitis: Analysis of Lymphocyte Subsets and Adhesion Molecule Expression
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Selective Recruitment of T-Cell Subsets to the Udder during Staphylococcal and Streptococcal Mastitis: Analysis of Lymphocyte Subsets and Adhesion Molecule Expression

机译：在葡萄球菌和链球菌性乳腺炎期间，将T细胞亚群选择性招募至乳房：淋巴细胞亚群和黏附分子表达的分析

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During bacterial infection of the bovine mammary gland, large numbers of leukocytes migrate into the udder, resulting in the establishment of a host response against the pathogen. Currently, the specific leukocyte populations mediating this immune response are not well defined. In the studies described here, we analyzed blood and milk from healthy cows and cows with naturally occurring mastitis to determine if distinct αβ and γδ T-lymphocyte subsets were involved in the response of the udder to a mastitis pathogen and if the type of mastitis pathogen influenced the subset composition of these responding leukocytes. Although blood samples from cows with confirmed staphylococcal and streptococcal mastitis were characterized by increased numbers of γδ T cells, the most dramatic changes in leukocyte distributions occurred in milk samples from these cows, with a 75% increase in αβ T-cell levels and a 100% increase in γδ T-cell levels relative to the levels in milk samples from healthy animals. Interestingly, the increase in αβ T-cell numbers observed in milk from cows with staphylococcal mastitis was primarily due to increased numbers of CD4+ T cells, while the increase in αβ T-cell numbers observed in cows with streptococcal mastitis was due to a parallel increase in both CD4+ and CD8+ T-cell numbers. The increased numbers of γδ T cells in milk from cows with staphylococcal and streptococcal mastitis were due to a selective recruitment of a distinct γδ T-cell subset (GD3.1+), while no change in the numbers of GD197+ γδ T cells was observed. We also analyzed adhesion protein expression on blood and milk leukocytes and found that, in comparison to the situation for healthy cows, L-selectin was down-regulated and CD18 was up-regulated on leukocytes from cows with mastitis. Thus, shedding of L-selectin and up-regulation of CD18 by neutrophils may provide a sensitive indicator of early inflammatory responses during bovine mastitis. Overall, these studies suggest that distinct αβ and γδ T-cell subsets are involved in the host defense of the udder against mastitis infection and that selective recruitment of these T-cell subsets depends on the infectious agent involved.
机译：在牛乳腺的细菌感染过程中，大量白细胞迁移到乳房中，导致建立了针对病原体的宿主反应。目前，介导这种免疫应答的特定白细胞群体尚不清楚。在此处描述的研究中，我们分析了健康母牛和自然发生乳腺炎的母牛的血液和牛奶，以确定乳房对乳腺炎病原体的反应是否涉及不同的αβ和γδT淋巴细胞亚群，以及乳腺炎病原体的类型影响了这些反应白细胞的亚组组成。尽管确诊为葡萄球菌和链球菌性乳腺炎的奶牛的血液样本以γδT细胞数量增加为特征，但这些奶牛的奶样中白细胞分布的变化最为显着，其中αβT细胞水平增加了75％，而乳汁中的100相对于健康动物的牛奶样品中的γδT细胞水平增加了％。有趣的是，在患有葡萄球菌性乳腺炎的母牛的牛奶中观察到的αβT细胞数量增加主要是由于CD4 + T细胞的数量增加，而在患有乳腺炎的奶牛中观察到的αβT细胞数量增加。链球菌性乳腺炎是由于CD4 + 和CD8 + T细胞数量同时增加所致。患有葡萄球菌和链球菌性乳腺炎的奶牛牛奶中γδT细胞数量的增加是由于选择性募集了独特的γδT细胞亚群（GD3.1 + ），而数量没有变化观察到GD197 + γδT细胞的表达。我们还分析了血液和牛奶白细胞上粘附蛋白的表达，发现与健康奶牛的情况相比，乳腺炎奶牛的白细胞上L-选择蛋白下调，CD18上调。因此，中性粒细胞的L-选择蛋白脱落和CD18上调可能提供了牛乳腺炎早期炎症反应的敏感指标。总体而言，这些研究表明，乳房的宿主防御乳腺炎感染涉及不同的αβ和γδT细胞亚群，而这些T细胞亚群的选择性募集取决于所涉及的感染因子。

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