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首页> 外文期刊>Infection and immunity >noxR3, a Novel Gene fromMycobacterium tuberculosis, Protects Salmonella typhimurium from Nitrosative and Oxidative Stress
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noxR3, a Novel Gene fromMycobacterium tuberculosis, Protects Salmonella typhimurium from Nitrosative and Oxidative Stress

机译：结核分枝杆菌的新基因noxR3保护鼠伤寒沙门氏菌免受亚硝化和氧化胁迫

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Reactive oxygen intermediates (ROI) and reactive nitrogen intermediates (RNI) produced by activated macrophages participate in host defense against the facultative intracellular pathogensMycobacterium tuberculosis and Salmonella typhimurium. To survive within macrophages, such pathogens may have evolved ROI and RNI resistance mechanisms. ROI resistance pathways have been intensively studied. Much less is known about the mechanisms of resistance to RNI. To identify possible RNI resistance genes inM. tuberculosis, a mycobacterial library was expressed inS. typhimurium and subjected to selection by exposure to the NO donor S-nitrosoglutathione (GSNO) in concentrations sufficient to kill the vast majority of nontransformed salmonellae. Among the rare surviving recombinants was a clone expressingnoxR3, a novel and previously anonymous M. tuberculosis gene predicted to encode a small, basic protein. Expression of noxR3 protected S. typhimuriumnot only from GSNO and acidified nitrite but also from H2O2. noxR3 is the third gene cloned from M. tuberculosis that has been shown to protect heterologous cells from both RNI and ROI. This suggests diversity in the repertoire of mechanisms that help pathogens resist the oxidative and nitrosative defenses of the host.
机译：活化的巨噬细胞产生的活性氧中间体（ROI）和活性氮中间体（RNI）参与宿主对胞内病原体结核分枝杆菌和鼠伤寒沙门氏菌的防御。为了在巨噬细胞内生存，这种病原体可能已经发展了ROI和RNI耐药机制。 ROI抵抗途径已得到深入研究。人们对RNI的抗药性知之甚少。为了鉴定 M中可能的RNI抗性基因。结核，在 S中表达了一个分枝杆菌文库。鼠伤寒沙门氏菌，并通过暴露于浓度足以杀死绝大多数未转化沙门氏菌的NO供体 S -亚硝基谷胱甘肽（GSNO）中进行选择。在幸存的稀有重组子中，有一个表达 noxR3 的克隆，该克隆是一种新型且以前匿名的 M。结核病基因预计会编码一种小的碱性蛋白质。受 noxR3 保护的 S的表达。鼠尾草不仅来自GSNO和酸化的亚硝酸盐，还来自H 2 O 2 。 noxR3 是从 M克隆的第三个基因。结核病已被证明可以保护异源细胞免受RNI和ROI的侵害。这表明机制的多样性有助于病原体抵抗宿主的氧化和亚硝化防御。

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