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Regulation of Brucella abortusCatalase

机译：布鲁氏菌流产过氧化氢酶的调节

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All aerobic organisms have mechanisms that protect against oxidative compounds. Catalase, peroxidase, superoxide dismutase, glutathione, and thioredoxin are widely distributed in many taxa and constitute elements of a nearly ubiquitous antioxidant metabolic strategy. Interestingly, the regulatory mechanisms that control these elements are rather different depending on the nature of the oxidative stress and the organism. Catalase is well documented to play an important role in protecting cells from oxidative stress. In particular, pathogenic bacteria seem to use this enzyme as a defensive tool against attack by the host. To investigate the significance of catalase in hostile environments, we made catalase deletion mutations in two different B. abortus strains and used two-dimensional gel analysis, survival tests, and adaptation experiments to explore the behavior and role of catalase under several oxidative stress conditions. These studies show that B. abortus strains that do not express catalase activity exhibit increased sensitivity to hydrogen peroxide. We also demonstrate that catalase expression is regulated in this species, and that preexposure to a sublethal concentration of hydrogen peroxide allows B. abortus to adapt so as to survive subsequent exposure to higher concentrations of hydrogen peroxide.
机译：所有有氧生物都有防止氧化的机制。过氧化氢酶，过氧化物酶，超氧化物歧化酶，谷胱甘肽和硫氧还蛋白广泛分布在许多分类单元中，构成了几乎普遍存在的抗氧化剂代谢策略的要素。有趣的是，取决于氧化应激和生物体的性质，控制这些元素的调节机制是相当不同的。充分证明过氧化氢酶在保护细胞免受氧化应激中起重要作用。尤其是，病原细菌似乎将这种酶用作防御宿主攻击的防御工具。为了研究过氧化氢酶在敌对环境中的重要性，我们在两个不同的流产双歧杆菌中产生了过氧化氢酶缺失突变，并使用了二维凝胶分析，存活测试和适应性实验来探索过氧化氢酶在几种氧化应激条件下的行为和作用。这些研究表明，不表达过氧化氢酶活性的流产芽孢杆菌菌株对过氧化氢的敏感性增加。我们还证明了过氧化氢酶的表达在该物种中受到调节，并且预先暴露于亚致死浓度的过氧化氢可使流产芽孢杆菌适应，从而在随后暴露于更高浓度的过氧化氢中存活下来。

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