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Serological Expression Cloning of Novel Immunoreactive Antigens of Babesia microti

机译：小巴贝虫新免疫反应抗原的血清学表达克隆

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Increased recognition of the prevalence of human babesiosis in the United States, together with rising concern about the potential for transmission of this infection by blood transfusion, has provided motivation to develop definitive serologic and molecular tests for the causative agent, Babesia microti. To develop more sensitive and specific assays for B. microti, we screened a genomic expression library with patient serum pools. This screening resulted in the identification of three classes of novel genes and an additional two novel, unrelated genes, which together encode a total of 17 uniqueB. microti antigens. The first class (BMN1-2 family) of genes encodes seven closely related antigens with a degenerate six-amino-acid repeat that shows limited homology toPlasmodium sp. merozoite and sporozoite surface antigens. A second class (BMN1-8 family) of genes encodes six related antigens, and the third class (BMN1-17 family) of genes encodes two related antigens. The two remaining genes code for novel and unrelated sequences. Among the three classes of antigens and remaining novel sequences, five were chosen to code for the most immunodominant antigens (BMN1-2, -9, -15, and -17 and MN-10). Western blot analysis with the resulting recombinant proteins indicated that these antigens were targets of humoral immune responses during B. microti infection in humans.
机译：在美国，人们对人杆状杆菌病的流行程度的日益认识，以及对通过输血传播这种感染的潜在可能性的日益关注，为推动对致病菌小巴贝氏体病的确定性血清学和分子学检测提供了动力。为了开发针对B. microti的更灵敏和特异的检测方法，我们筛选了具有患者血清库的基因组表达文库。该筛选导致鉴定出三类新基因和另外两个新的，不相关的基因，它们一起编码总共17个uniqueB。微量抗原。第一类（BMN1-2家族）基因编码七个密切相关的抗原，具有简并的六氨基酸重复序列，该重复序列与疟原虫的同源性有限。裂殖子和子孢子的表面抗原。第二类（BMN1-8家族）基因编码六个相关抗原，第三类（BMN1-17家族）基因编码两个相关抗原。剩下的两个基因编码新的和无关的序列。在三类抗原和其余新序列中，选择了五种来编码最具免疫优势的抗原（BMN1-2，-9，-15和-17和MN-10）。用所得重组蛋白进行的蛋白质印迹分析表明，这些抗原是人类小肠芽孢杆菌感染期间体液免疫反应的靶标。

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