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Genetic Diversity of the Capsular Polysaccharide C Biosynthesis Region of Bacteroides fragilis

机译：脆弱拟杆菌的荚膜多糖C生物合成区的遗传多样性

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A genetic approach was used to assess the heterogeneity of the capsular polysaccharide C (PS C) biosynthesis locus ofBacteroides fragilis and to determine whether distinct loci contain genes whose products are likely to be involved in conferring charged groups that enable the B. fragilis capsular polysaccharides to induce abscesses. A collection of 50 B. fragilis strains was examined. PCR analysis demonstrated that the genes flanking the PS C biosynthesis region are conserved, whereas the genes within the loci are heterogeneous. OnlycfiA + B. fragilis strains, which represent 3% of the clinical isolates of B. fragilis, displayed heterogeneity in the regions flanking the polysaccharide biosynthesis genes. Primers were designed in the conserved regions upstream and downstream of the PS C locus and were used to amplify the region from 45 of the 50 B. fragilis strains studied. Fourteen PS C genetic loci could be differentiated by a combination of PCR and extended PCR. These loci ranged in size from 14 to 26 kb. Hybridization analysis with genes from the PS C loci of strains 9343 and 638R revealed that the majority of strains contain homologs ofwcgC (N-acetylmannosamine dehydrogenase),wcfF (putative dehydrogenase), and wcgP(putative aminotransferase). The data suggest that the synthesis of polysaccharides that have zwitterionic characteristics rendering them able to induce abscesses is common in B. fragilis.
机译：遗传方法用于评估脆弱拟杆菌（Bacteroides fragilis）荚膜多糖C（PS C）生物合成位点的异质性，并确定不同的基因座是否包含其产物可能涉及赋予带电基团的基因。启用 B。脆弱的荚膜多糖诱导脓肿。 50个 B的集合。检查了脆弱的菌株。 PCR分析表明，PS C生物合成区域两侧的基因是保守的，而基因座内的基因是异质的。仅 cfiA + B。脆弱菌菌株，占 B临床分离株的3％。脆弱类植物在多糖生物合成基因侧翼区域表现出异质性。在PS C基因座上游和下游的保守区域设计引物，并用于从50个B的45个区域扩增该区域。研究了脆弱类的菌株。通过组合PCR和扩展PCR可以区分14个PS C遗传基因座。这些基因座的大小在14到26 kb之间。对来自9343和638R菌株PS C基因座的基因进行的杂交分析表明，大多数菌株含有 wcgC （ N -乙酰甘露糖胺脱氢酶）， wcfF < / em>（可能的脱氢酶）和 wcgP （可能的氨基转移酶）。数据表明，具有两性离子特性使其能够诱导脓肿的多糖的合成在B中很常见。脆弱的。

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